山东农业科学2016，Vol.48Issue(5)：9-13,5.DOI:10.14083/j.issn.1001-4942.2016.05.003
库尔勒香梨组织培养的研究
Research on Tissue Culture of Korla Fragrant Pear
摘要
Abstract
Korla fragrant pear has soft flesh and delicious flavor,which is one of the important agricul-tural exports of China.While,there are problems of low fruit setting rate and unstable yield of Korla fragrant pear.Improving the productivity traits of fruit tree by genetic transformation based on tissue culture is a signifi-cant method for high efficiency breeding.In this paper,the in vitro tissue culture method of Korla fragrant pear was established using tender buds as explants.The effects of disinfection time of 0.1% HgCl2 solution on sterilizing effect and browning of explants were studied and the optimum multiplication medium and rooting me-dium were explored.The results showed that during the tissue culture process of Korla fragrant pear,the opti-mum disinfection time of 0.1% HgCl2 was 5 minutes;the optimum multiplication medium was MS +1.5 mg/L 6 -BA +0.1 mg/L IBA +1.5 mg/L GA3;and the optimum rooting medium was 1 /2MS +0.05 g/L AC +1.0 mg/L IBA.关键词
库尔勒香梨/芽/组织培养/消毒时间/增殖/生根Key words
Korla fragrant pear/Bud/Tissue culture/Disinfection time/Multiplication/Rooting分类
农业科技引用本文复制引用
刘小芳,冯建荣,梁晓桐,吕文娟,李文慧,樊新民..库尔勒香梨组织培养的研究[J].山东农业科学,2016,48(5):9-13,5.基金项目
国家自然科学基金项目 （）
