山东农业科学2016，Vol.48Issue(5)：62-66,5.DOI:10.14083/j.issn.1001-4942.2016.05.017
响应面法优化生防枯草芽孢杆菌B579固相发酵工艺
Production Optimization of Biocontrol Bacillus subtilis B579 by Solid -State Fermentation Using Response Surface Methodology
摘要
Abstract
To enhance the fermentation level of biocontrol Bacillus,the solid -state fermentation condi-tions of Bacillus subtilis B579 were optimized using response surface methodology to establish the related tech-nology system.Firstly,the Plackett -Burman experiment was applied to screen the importan influential fac-tors,and then the path of steepest ascent was used to approach the biggest region of the cell yield.Moreover, the Box -Benhnken experimental design and response surface methodology were employed to get the regression model,which was proved through regression analysis and practice verification.The results showed that the three factors affecting fermentation production were beef extract,corn meal and soluble starch.The fermenta-tion production reached the highest as 1.93 ×10 11 cfu /g,when the content of beef extract,corn meal and sol-uble starch were 15.1,10.3 and 9.14 g/kg respectively.Under the optimized fermentation conditions,the solid fermentation production of biocontrol Bacillus increased by 15 times compared to the liquid fermentation. The regression model was verified to be accurate and reliable.关键词
生防芽孢杆菌/固相发酵/响应面法Key words
Biocontrol Bacillus/Solid -state fermentation/Response surface methodology分类
农业科技引用本文复制引用
孙冰冰,杨秀荣,郝永娟,李伟,魏军,田涛..响应面法优化生防枯草芽孢杆菌B579固相发酵工艺[J].山东农业科学,2016,48(5):62-66,5.基金项目
天津市农业科学院院长基金项目“以农业副产物为基质生防枯草芽孢杆菌 B579固相发酵技术研究”（13011）；天津市农业科技成果转化与推广项目“土壤病害复合微生物杀菌剂的中试及示范”（201501040）；天津市科技支撑项目“瓜类蔬菜土传病害安全防控技术集成与示范”（13ZCZDNC00100）；天津市科技计划项目“枯草芽孢杆菌 B579应用技术示范与推广” （）
