山东农业科学2016，Vol.48Issue(5)：131-136,6.DOI:10.14083/j.issn.1001-4942.2016.05.034
同时测定6种柿叶黄酮类物质方法的建立
Establishment of Simultaneous Detection Method of Six Flavonoids from Persimmon Leaves
摘要
Abstract
The HPLC method for simultaneous detection of 6 flavonoids from persimmon leaves was es-tablished by exploring the detection conditions such as wavelength,mobile phase and column temperature. The 6 flavonoids were lutin,kaempferol,isorhamnetin,quercetin,isoquercitrin and astragalin.The results showed that the 6 flavonoid standards could be detected simultaneously under the following conditions:using C18 as stuffing bulking agent,carbinol as mobile phase A and 0.5% acetic acid as mobile phase B;the sam-ples were gradiently eluted at the ratios of phase A∶B as 20∶80 in 0 ~100 minutes and 40∶60 after 105 mi-nutes;and the detection was carried out with 360 nm of wavelength,35℃ of column temperature and 1.0 mL/min of volume flow rate.The detection method was verified with the leaves of two persimmon varieties, and the expected results were obtained,which indicated that the established method was feasible.关键词
柿叶/黄酮类物质/高效液相色谱/同时检测Key words
Persimmon leaves/Flavonoid/HPLC/Simultaneous detection分类
农业科技引用本文复制引用
安淼,余贤美,王洁,刘加芬,艾呈祥..同时测定6种柿叶黄酮类物质方法的建立[J].山东农业科学,2016,48(5):131-136,6.基金项目
“十二五”农村领域国家科技支撑计划项目“涩柿和甜柿高效生产关键技术研究与示范”（2013BAD14B0504）；山东省农业良种工程项目“优质、高抗、高档干果新品种选育”（2014lz14）；山东省农业科学院青年科研基金项目“柿抗炭疽病相关基因的克隆与功能分析” （）
