山东医药2016，Vol.56Issue(14)：1-3,3.DOI:10.3969/j.issn.1002-266X.2016.14.001
骨髓间充质干细胞旁分泌效应对胰腺β细胞增殖、凋亡及分泌功能的影响
Influence of paracrine effects of bone mesenchymal stem cells on proliferation, apoptosis and secretion function of pancreatic βcell line
摘要
Abstract
Objective To investigate the influence of paracrine effects of bone mesenchymal stem cells (BMSCs)on the proliferation,apoptosis and insulin secretion of pancreaticβcell line INS-1 cells.Methods The BMSCs were cultured and BM-SCs conditioned media (BMSCs-CM)were obtained by culturing BMSCs with rat pancreatic extracts (RPE)which simulated the pancreatic microenvironment.The INS-1 cells were divided into groups A,B and C,which were cultured with BMSCs-CM,con-ditioned media without RPE and RPMI 1640 culture solution,respectively.The influence of BMSCs-CM on INS-1 cell prolifera-tion was analyzed by MTT.The cell proliferation rate was calculated.The effects of BMSCs-CMon the INS-1 cell apoptosis were measured by AnnexinⅤ/PI double staining.The rate of apoptosis was counted.The insulin concentration of INS-1 cells was ana-lyzed by radioimmunoassay (RIA).Results The cell proliferation rate in the group A was higher than that in group B and C (P<0.05).The apoptosis rate in the group A was lower than that in group B and C (P<0.05).The insulin concentration in the group A was higher than that in group B and C at the concentration of 5.6 mmol/L and 20.0 mmol/L (all P<0.05).Conclu-sions The incubation of BMSCs-CMcan stimulate the INS-1 cell proliferation,inhibit the apoptosis,and promote the insulin se-cretion.The above actions of BMSCs may be achieved by paracrine effects.关键词
干细胞,骨髓/胰腺β细胞/细胞因子/凋亡/增殖Key words
stem cells,myeloid/pancreatic βcells/cytokines/apoptosis/proliferation分类
医药卫生引用本文复制引用
许聿新,井庆平,赵翠红..骨髓间充质干细胞旁分泌效应对胰腺β细胞增殖、凋亡及分泌功能的影响[J].山东医药,2016,56(14):1-3,3.基金项目
山东省淄博市科学技术发展计划项目（2012GG01259）。 （）
