山东医药2016，Vol.56Issue(14)：4-6,3.DOI:10.3969/j.issn.1002-266X.2016.14.002
葡萄多酚对糖尿病大鼠膀胱功能的保护作用及其机制探讨
Protective effect of grape-seed proanthocyanidin extract on bladder function of rats with diabetes mellitus
摘要
Abstract
Objective To investigate the effect and mechanism of grape-seed proanthocyanidin extract (GSPE )on bladder function of rats with diabetes mellitus (DM).Methods Thirty female Wistar rats was randomly divided into 3 groups:control group,DMgroup and GSPE group,and each group consisted of 10 rats.Intraperitoneal injection of strepto-zotocin (STZ)was used to construct the model of diabetes in DM group and GSPE group.The same dose of sodium citrate buffer was used in the control group.Rats in the GSPE group were given 250 mg/kg of GSPE by oral gavage once a day for 8 weeks.The control group and DM group were treated with the same amount of normal saline.Urodynamic method was used to examine the bladder pressure of the rats.The bladder histopathological changes were observed by HE staining.The quantity of MDA and the activities of superoxide dismutase (SOD)and GSH-Px was examined by spectrophotometry.Re-sults Compared with the control group,the bladder contraction pressure in the DM group and GSPE group was decreased (P<0.01).However,the bladder contraction pressure in the GSPE group was higher than that in the DM group (P<0.01).According to HE staining,the bladder tissue became thin,the detrusor was disordered and the infiltrating inflam-matory cells were increased in the DM group as compared with those of the control group.The bladder tissue became thick and the infiltrating inflammatory cells were decreased in the GSPE group as compared with those of the DM group.Com-pared with the DM group,the level of MDA was lower and the levels of SOD and GSH-Px were higher in the GSPE group (P<0.01 or P<0.05).Conclusion GSPE has protective effect on diabetic bladder dysfunction,and its mechanism may be associated with the increased oxidative stress injury of the bladder tissues.关键词
糖尿病/膀胱功能障碍/葡萄多酚/氧化应激Key words
diabetes mellitus/bladder dysfunction/grape-seed proanthocyanidin extract/oxidative stress分类
医药卫生引用本文复制引用
高兆允,陈守臻,史本康..葡萄多酚对糖尿病大鼠膀胱功能的保护作用及其机制探讨[J].山东医药,2016,56(14):4-6,3.基金项目
国家自然科学基金资助项目（81470987）。 （）
