山东医药2016，Vol.56Issue(14)：11-13,3.DOI:10.3969/j.issn.1002-266X.2016.14.004
FOXA 1对人乳头状甲状腺癌细胞增殖与细胞周期的影响及其机制探讨
Effects of FOXA1 on proliferation and cell cycle of human papillary thyroid carcinoma cells
摘要
Abstract
Objective To observe the effect of forkhead box protein A1 (FOXA1 )on the proliferation and cell cycle of human papillary thyroid carcinoma TPC1 cells and to investigate its mechanism.Methods TPC1 cells were divided into two groups which were separately transfected with Flag-vector (empty vector group)and Flag-FOXA1 (FOXA1 group). The proliferation activity of the two groups of TPC1 cells was detected by MTT method after transfection for 12 h,24 h and 36 h,and cell cycle was detected by flow cytometry at 24 h.The mRNA and protein expression of p27 Kip1 ,cyclin-de-pendent kinase (CDK2)and CyclinD1 was detected by real-time PCR and Western blotting at 24 h after transfection of Flag-FOXA1 .Results In the FOXA1 group,the cell proliferation was promoted,cells in the G1 phase decreased,but cells in the S phase increased.Compared with the empty vector group,the expression of p27 Kip1 mRNA and protein was decreased and the expression of CyclinD1 and CDK2 mRNA and protein was increased.Conclusions FOXA1 can pro-mote the cell proliferation and increase the cells in the S period.The mechanism may be related to the down-regulation of p27 Kip1 and up-regulation of CDK2 and CyclinD1 expression.关键词
叉头框蛋白A1/细胞增殖/细胞周期/甲状腺癌Key words
forkhead box protein A1/cell proliferation/cell cycle/thyroid carcinoma分类
医药卫生引用本文复制引用
王月娥,王春玉..FOXA 1对人乳头状甲状腺癌细胞增殖与细胞周期的影响及其机制探讨[J].山东医药,2016,56(14):11-13,3.基金项目
国家自然科学基金资助项目（31401115）。 （）
