山东医药2016，Vol.56Issue(14)：21-24,4.DOI:10.3969/j.issn.1002-266X.2016.14.007
利妥昔单抗对CIK细胞杀伤活性及细胞毒作用的影响
Effect of rituximab on killing activity and cytotoxicity of cytokine-induced killer cells
摘要
Abstract
Objective To investigate the effect of rituximab on killing activity and cytotoxicity of cytokine-induced killer (CIK)cells and to provide basis for the clinical application of rituximab.Methods CIK cells from peripheral blood of healthy donors were prepared and then were divided into the control group,observation group 1 and observation group 2. The observation group 1 and observation group 2 were respectively added with 5 ×104 ,10 ×104 μg/L rituximab and the control group was not treated.On day 14,the expression of CD3 +,CD3 +CD56 +was analyzed by flow cytometry,the lev-els of tumor necrosis factor-α(TNF-α)and interferonγ(INF-γ)were detected by enzyme-linked immunosorbent method, and the expression of CD314,granzyme B and perforin in the CIK cells of the three groups was detected by flow cytometry. In the three groups,three holes of CIK cells were reset to co-culture with K562 cells and SU-DHL2 cells with the ratios of E/T (10∶1,20∶1 and 40∶1),and then the cytotoxicity of the CIK cells against K562 or SU-DHL2 cell lines were meas-ured using MTT.Results The phenotypes of CD3 +,CD3 +CD56 +had no statistical difference among these three groups (P>0.05).The levels of INF-γand TNF-αin the supernatant of the observation group 1 and observation group 2 were higher than that of the control group (P<0.05).The expression of CD314,granzyme B and perforin in the observation group 1 and observation group 2 were higher than that of the control group (all P<0.05).Cytotoxicity against SU-DHL4 or K562 cells at different ratio of E/T (10∶1,20∶1 and 40∶1)in the observation group 1 and observation group 2 were higher than that of the control group (all P<0.05).Conclusion Rituximab can improve the killing activity of CIK cells and has cytotoxic effect on the myeloid leukemia cells and B-lymphoma cells.关键词
利妥昔单抗/细胞因子诱导杀伤细胞/白血病/淋巴瘤Key words
rituximab/cytokine-induced killer cells/leukemia/lymphoma分类
医药卫生引用本文复制引用
李青,邓琦,赵明峰,李玉明..利妥昔单抗对CIK细胞杀伤活性及细胞毒作用的影响[J].山东医药,2016,56(14):21-24,4.基金项目
天津市卫生局科技基金资助项目（2014KZ021）；天津市卫生行业重点攻关项目（13KG106）。 （）
