山东医药2016，Vol.56Issue(21)：10-12,3.DOI:10.3969/j.issn.1002-266X.2016.21.004
miR-206对高磷诱导大鼠血管平滑肌细胞钙化的影响及机制
Effect of miR-206 on high phosphorus-induced calcification in rat vascular smooth muscle cells
摘要
Abstract
Objective To investigate the effect of microRNA-206 (miR-206)on β-glycerophosphate (β-GP)-in-duced calcification in rat vascular smooth muscle cells (VSMCs).Methods Aortal VSMCs were primarily cultured and i-dentified in vitro.VSMCs were divided into five groups:control group,mimics negative control group,miR-206 mimic group,inhibitor negative control group and miR-206 inhibitor group.Each group was transfected by lipofectamine2000.Af-ter 48-hour transfection,each group was stimulated with 10 mmol/Lβ-GP to induce calcification of VSMCs.Observing cal-cium deposition with Alizarin red staining while detecting the alkaline phosphate (ALP)activity and the expression of con-nexin 43 (Cx-43)respectively by Trace enzyme standard method and immunofluorescence method after induction for 4 days.Results After transfection 48 h,VSMCs were treated with β-GP for 4 days.Compared with the negative control group,the calcium deposition decreased in the miR-206 mimic group and increased in the miR-206 inhibitor group;the ALP activity and the expression of Cx43 decreased in the miR-206 mimic group and increased in the miR-206 inhibitor group (all P <0.05).Conclusions miR-206 may inhibit β-GP-induced calcification of VSMCs by regulating Cx43.关键词
微小RNA-206/β-甘油磷酸钠/血管平滑肌细胞/钙化/大鼠Key words
microRNA-206/β-glycerophosphate/vascular smooth muscle cells/calcification/rats分类
医药卫生引用本文复制引用
邵娟,吴基良,李敏才..miR-206对高磷诱导大鼠血管平滑肌细胞钙化的影响及机制[J].山东医药,2016,56(21):10-12,3.基金项目
国家自然科学基金资助项目（81270355）；湖北省自然科学基金资助项目（2014CFB211）。 （）
