上海针灸杂志2016，Vol.35Issue(6)：636-638,3.DOI:10.13460/j.issn.1005-0957.2016.06.0636
针刺关元穴对寒凝证类痛经大鼠扭体反应和血管舒缩物质的影响
Effect of Acupuncture at Guanyuan (CV4) on Writhing Response and Vasomotor Substances in Rats with Dysmenorrhea Due to Coagulated Cold Syndrome
摘要
Abstract
Objective To observe the analgesic effect of acupuncture at Guanyuan (CV4) and its effect on vasomotor substances in rats with dysmenorrhea due to coagulated cold syndrome. Method The coagulated-cold dysmenorrhea rat model was developed by Estrodiol benzoate and Oxytocin injectin plus physical freezing. The writhing response (writhing latency, writhing frequency, and writhing score) was observed, and the contents of TXB2 and 6-keto-PGF1a were detected by using enzyme-linked immunosorbent assay (ELISA). Result Compared with the saline water group, the writhing latency was significantly shortened, the writhing frequency was significantly increased, and the writhing score was more significantly increased in the model group (P<0.01);compared with the model group, the writhing latency was significantly prolonged, the writhing frequency was decreased, and the writhing score was significantly lower in the acupuncture group (P<0.05, P<0.01). Compared with saline water group, the content of plasma 6-keto-PGF1a was significantly lower (P<0.05) and the content of plasma TXB2 showed an increasing tendency (P>0.05) in the model group. Compared with the model group, the content of plasma 6-keto-PGF1a showed an increasing tendency (P>0.05) and the content of plasma TXB2 showed a decreasing tendency (P>0.05) in the acupuncture group. Conclusion The vasomotor substances are obviously disordered in the blood of cold-coagulated dysmenorrhea rat models. Acupuncture at Guanyuan can improve the writhing response and release pain, and meanwhile positively regulate the vasomotor substances such as TXB2 and 6-keto-PGF1a. The vasomotor substances are plausibly one of the major substances in the action of acupuncture in preventing and treating dysmenorrhea.关键词
针刺/穴,关元/寒凝证/痛经/扭体反应/血管舒缩物质/大鼠Key words
Acupuncture/Point, Guanyuan(CV4)/Coagulated cold syndrome/Dysmenorrhea/Writhing response/Vasomotor substance/Rats分类
医药卫生引用本文复制引用
王洋,杨佳敏,沈小雨,郭孟玮,赵雅芳,嵇波,任晓暄,张露芬,朱江,李凯歌,谭莉华,罗丽,李晓泓,赵鹏飞,章庆庆,朱世鹏,张玲,申松希,齐丹丹..针刺关元穴对寒凝证类痛经大鼠扭体反应和血管舒缩物质的影响[J].上海针灸杂志,2016,35(6):636-638,3.基金项目
国家重点基础研究发展计划项目(2012CB518506,2006CB504503,2005CB523308) （2012CB518506,2006CB504503,2005CB523308）
国家自然科学基金面上项目(30973793) （30973793）
教育部博士点基金项目(20090013110005) （20090013110005）
