上海针灸杂志2016，Vol.35Issue(6)：738-741,4.DOI:10.13460/j.issn.1005-0957.2016.06.0738
针刺联合黄芪多糖对db/db小鼠胰岛β细胞Bcl-2蛋白表达的影响
Effect of Acupuncture plus Astragalus Polysaccharide on the Expression of Bcl-2 Protein in Islet Cells in db/db Mice
摘要
Abstract
Objective To investigate the effect of acupuncture plus astragalus polysaccharide on the expression of Bcl-2 protein in pancreatic islet b cells in db/db mice. Method C57BL/Ksj-db/db mice as an animal model of spontaneous type 2 diabetes were selected for this experiment. Five-week-old db/db mice were randomized into model, acupuncture, medication and acupuncture+medication groups. Meanwhile, db/m mice were selected as a normal group. The acupuncture group received acupuncture at points Housanli (equivalent to Zusanli, ST36), Neiting(ST44) and Yishu(Extra) and the medication group, an oral gavage of astragalus polysaccharide (1400 mg/kg). Both groups were treated once daily, for 12 consecutive weeks. After the end of experiment, blood glucose, insulin and resistin were measured, and the expression of Bcl-2 protein in islet b cells was determined by immunohistochemical method. Result Blood glucose, insulin and resistin levels were significantly lower in the acupuncture+medication, acupuncture and medication groups than in the model group. They were significantly lower in the acupuncture+medication group than in the acupuncture and medication groups and significantly lower in the acupuncture group than in the medication group. The expression of Bcl-2 protein in islet b cells was higher in the medication, acupuncture and acupuncture+medication groups than in the model group; there was a statistically significant difference (P<0.01). The expression of Bcl-2 protein was higher in the acupuncture+medication groups than in the medication group (P<0.01) and it was basically the same in the medication group as in the acupuncture group; there was no statistically significant difference (P>0.05). Conclusion Acupuncture plus astragalus polysaccharide can significantly reduce blood glucose and serum insulin and resistin levels and increase the expression of Bcl-2 protein in islet b cells to effectively inhibit apoptosis in islet b cells in db/db mice. Its effect is better than that of acupuncture alone or medication.关键词
针刺/黄芪多糖/糖尿病,非胰岛素依赖型/β细胞/B淋巴细胞瘤蛋白/小鼠Key words
Acupuncture/Astragalus polysaccharide/Diabetes, non-insulin dependent/βcell/B-lymphocyte tumor protein/Mice分类
医药卫生引用本文复制引用
张文奎,李茜,宫翠红,孙志..针刺联合黄芪多糖对db/db小鼠胰岛β细胞Bcl-2蛋白表达的影响[J].上海针灸杂志,2016,35(6):738-741,4.基金项目
江苏省针灸学重点实验室(针药结合实验室)开放课题(KJA200904) （针药结合实验室）
