中国生态农业学报2016，Vol.24Issue(6)：736-743,8.DOI:10.13930/j.cnki.cjea.151212
玉米秸秆生物炭对褐土微生物功能多样性及细菌群落的影响
Effect of corn-stalk biochar on soil microbial functional diversity and bacterial community in cinnamon soils
摘要
Abstract
Biochar application was considered as an effective way of carbon sequestration of soil. It has been known to improve both soil water and nutrient holding capacity. As a key indicator of soil fertility, microorganism plays an important role in soil ecosystem. It was necessary to understand the influence of biochar application on microbial community and functional diversity in calcareous cinnamon soils for rational utilization of biochar in cinnamon soils. A pot experiment with five biochar application levels [0 g·kg-1 (as control), 5 g·kg-1, 10 g·kg-1, 30 g·kg-1 and 60 g·kg-1] was conducted to investigate the response of soil microbial activity and diversity, using Biolog analysis and high-throughput sequestration (HiSeq). The results showed that the average rate of color change (AWCD) decreased with increasing biochar application level, and the AWCD values were in the order of 5 g·kg-1≈ control > 10 g·kg-1> 30 g·kg-1 > 60 g·kg-1 treatments. There was no significant difference between control and 5 g·kg-1 treatments in terms of AWCD. The soil microbial community diversity index (H′) and richness index (S) also decreased, but evenness index (E) increased with increasing biochar application level. Compared with the control, biochar application rates at 5 g·kg-1, 10 g·kg-1, 30 g·kg-1 and 60 g·kg-1 increasedH′by 0.16%,-0.88%,-3.14% and-11.09%; increasedS by-2.82%,-11.27%,-18.31% and-47.89%; and increasedE by 1.14%, 3.00%, 3.73% and 13.76%; respectively. Principal component analysis showed that carbon substrate utilization of soil microbial community was significantly affected by 10 g·kg-1, 30 g·kg-1 and 60 g·kg-1 biochar application. HiSeq analysis showed that soil bacterial OTU enhanced significantly by 1.09%, 5.26% and 24.42%, respectively, in 10 g·kg-1, 30 g·kg-1 and 60 g·kg-1biochar treatments, meanwhile, the richness index (Chao1) was increased by 5.73%, 10.21% and 37.68%, respectively, compared with that of control. Biochar enhanced the abundance of bacteria and decreasedthe evenness of soil bacterial distribution. Proteobacteria abundance was significantly increased by 32.3%, 21.1% and 16.7%, respectively, under 10 g·kg-1, 30 g·kg-1, 60 g·kg-1biochar treatment, while Bacteroidetesabundance decreased obviously by 22.1%, 55.3%, 66.8% and 50.5%, respectively, compared with those of control. The results indicated that the rational dosage of biochar application was not more than 5 g·kg-1 (soil dry) in calcareous cinnamon soil to maintain the microbial community structure and activity.关键词
生物炭/石灰性褐土/微生物活性/微生物多样性/Biolog/高通量测序/细菌群落Key words
Biochar/Calcareous cinnamon soil/Microbial activity/Microbial diversity/Biolog/High-throughput sequencing/Bacterial community分类
农业科技引用本文复制引用
武爱莲,丁玉川,焦晓燕,王劲松,董二伟,郭珺,王浩..玉米秸秆生物炭对褐土微生物功能多样性及细菌群落的影响[J].中国生态农业学报,2016,24(6):736-743,8.基金项目
山西省农业科技攻关项目(20130311009-2)和山西省国际科技合作项目(2014081040)资助 This study was supported by the Agricultural Science and Technology Research Project of Shanxi Province (20130311009-2) and the International Science and Technology Cooperation Project of Shanxi Province (2014081040). （20130311009-2）
