生物加工过程2016，Vol.14Issue(3)：12-16,5.DOI:10.3969/j.issn.1672-3678.2016.03.003
中高温油藏内源微生物厌氧激活
Anaerobic activation of indigenous microorganism in the middle and high temperature reservoir
摘要
Abstract
In order to define the characteristics of gas production by indigenous microorganism under anaerobic activation,11 blocks of middle and high temperature reservoir were selected to research in the Shengli Oilfield,which temperature ranges were 55 to 65 ℃,65 to 79 ℃ and 79 to 95 ℃. Indigenous microorganism could be activated to produce gas below 79℃ under simulated reservoir condition,whereas no significant methane gas was produced when temperature was above 79 ℃. The maximum methane production rate was up to 1 500 μmol/( g·d ) when H2/CO2 was used as carbon source, significantly higher than those of sodium acetate and starch. Furthermore,Methanobacterium was activated to become the dominant microflora under anaerobic condition in Zhengli Zhuang Zhengnan block,which favored to produce methane gas in the reservoir.Before anaerobic activation,the dominant bacteria were Pseudomonas in the samples and the dominant bacteria were changed under different activation conditions.H2/CO2 and starch can activate Thermotoga, whereas sodium acetate mainly activated Deferribacter in oil wells. In addition,the dominant flora of different oil wells tended to be identical in the block, but there were significant differences in the dominant microflora between oil well and water well. Our findings on the anaerobic activation of the internal microorganism could serve reference for further enhancing the effect of microbial oil recovery.关键词
内源微生物/厌氧激活/甲烷气/群落结构Key words
indigenous microorganism/anaerobic activation/methane gas/community structure分类
生物科学引用本文复制引用
冯云,段传慧,林军章,孙刚正..中高温油藏内源微生物厌氧激活[J].生物加工过程,2016,14(3):12-16,5.基金项目
国家高技术研究发展计划（863计划） （）
