生物加工过程2016，Vol.14Issue(3)：23-26,32,5.DOI:10.3969/j.issn.1672-3678.2016.03.005
内源微生物驱油功能菌定量化分析技术
Quantification of bacteria responsible for endogenous microbial enhanced oil recovery
摘要
Abstract
We used the rapid SYBR� Green fluorescent quantitative real⁃time PCR technique to detect lipopeptide producing bacteria and methanogenic archaea present in the reservoir. The recombinant plasmid containing srfA and mcrA gene was used as standards to generate a standard curve for lipopeptide producing bacteria and methanogenic archaea. The sensitivity, reproducibility and specificity of the technique were evaluated.The correlation coefficient(R2) of the standard curve was above 0. 99 and the amplification efficiency reached 100%. To detect unknown samples, the detection limit of the method reached 10 copies/μL.Water samples collected in the oil well Z3⁃X24 located in Zhan3 block of Shengli oilfield were detected using the technique.The concentration of lipopeptide producing bacteria increased to 104 copies/μL in the early stage of endogenous activation, while the concentration of methanogenic archaea increased to 10 copies/μL in the late stage of endogenous activation. The data confirmed there was a good successive ruler between aerobic and anaerobic bacteria in the process of MEOR.We found a correlatin between these two functional bacteria dynamics and the oil production. These results are essential for understanding oil⁃displacement mechanism of indigenous MEOR and providing scientific guidance to the performance of MEOR technology in the future.关键词
产脂肽菌/产甲烷菌/定量化检测Key words
lipopeptide producing bacteria/methanogenic archaea/quantitative detection分类
能源科技引用本文复制引用
胡婧,吴晓玲..内源微生物驱油功能菌定量化分析技术[J].生物加工过程,2016,14(3):23-26,32,5.基金项目
国家高技术研究发展计划（863计划） （）
