生物加工过程2016,Vol.14Issue(3):39-45,7.DOI:10.3969/j.issn.1672-3678.2016.03.008
中高温油藏内源微生物好氧激活技术研究
Aerobic activation of endogenous microorganisms in middle and high temperature reservoir
摘要
Abstract
In order to study the endogenous microbial aerobic activation characteristics in middle and high temperature reservoir,12 typical blocks in high temperature reservoirs in Shengli Oilfield were selected to carry out the aerobic activation evaluation.Three reservoir temperature range were selected,namely from 55 to 65 ℃,65 to 79 ℃ and 79 to 95 ℃.6 different carbon sources were selected as the main activator:different activator formulation was prepared.Under the simulated reservoir conditions of aerobic activation evaluation,we found that the additional nutrient agent of corn syrup, molasses and yeast powder could effectively activate the functional strain below 79 ℃ oil reservoir blocks,the cell density could reach 3× 109 cell/mL.The emulsifying and dispersing effect of crude oil was obvious. In oil reservoir blocks above 79 ℃,the activation effect and emulsifying effect of crude oil were generally worse than that in 79 ℃ oil reservoir blocks.The cell density and emulsifying effect after activation show a positive correlation in each block center,whereas the surface tension and emulsifying effect had no obvious correlation. The surface tension of the solution after aerobic activation was reduced from 65 mN/m to 47 mN/m. The dominant bacteria after aerobic activation were mainly Geobacillus and Bacillus thermoamylovorans.关键词
中高温油藏/内源微生物/微生物驱油/好氧激活Key words
middle and high temperature reservoir/endogenous microorganism/microbial oil displacement/aerobic activation分类
能源科技引用本文复制引用
王静,段传慧,李彩风,高光军..中高温油藏内源微生物好氧激活技术研究[J].生物加工过程,2016,14(3):39-45,7.基金项目
国家高技术研究发展计划(863计划) ()