生物加工过程2016，Vol.14Issue(3)：46-52,7.DOI:10.3969/j.issn.1672-3678.2016.03.009
枯草芽胞杆菌M15101强化内源微生物驱油模拟实验
Enhancement of indigenous microbial flooding by exogenous Bacillus subtilis
摘要
Abstract
We used Bacillus subtilis M15⁃10⁃1 as the research object. Bacillus subtilis M15⁃10⁃1 is separated from the reservoir environment and can produce lipopeptide. We explored the exogenous surfactant producing bacteria to strengthen endogenous microbial flooding reservoirs in the process of crude oil emulsion dispersion effect and the interaction among bacterial flora. Compatibility experiments showed that Bacillus subtilis M15⁃10⁃1 and the oil reservoir functional bacteria, Dietzia sp. ZQ⁃4, Rhodococcus sp. M, and Enterobacter cloacae T⁃1 has good compatibility. Fluorescence quantitative PCR and high⁃throughput sequencing analysis showed that Bacillus subtilis M15⁃10⁃1 was the structure of reservoir indigenous microorganism without adverse impact. Activation experiment in laboratory and meteorological chromatography analysis showed that Bacillus subtilis M15⁃10⁃1 could significantly improve the oil water activation in early oil phase emulsifying dispersion effect and promote the petroleum hydrocarbon degradation.This method provides the foundation for subsequent field test.关键词
枯草芽胞杆菌/生物强化/内源微生物/微生物驱油/原油乳化Key words
Bacillus subtilis/bio-augmentation/indigenous micro-organisms/microbial flooding/oil emulsification分类
能源科技引用本文复制引用
李燕淑,李彦,高配科,代学成,田会梅,刘雪莲,常宁,李国强,马挺..枯草芽胞杆菌M15101强化内源微生物驱油模拟实验[J].生物加工过程,2016,14(3):46-52,7.基金项目
国家高技术研究发展计划（863计划）（2013AA064402）；国家自然科学基金 （）
