生物加工过程2016，Vol.14Issue(3)：68-74,7.DOI:10.3969/j.issn.1672-3678.2016.03.013
鼠李糖脂产生菌M76在模拟油藏条件下的培养基优化
Optimization of culture medium for rhamnolipid producing bacterial strain M7-6 under simulated oil reservoirs conditions
摘要
Abstract
Based on a rhamnolipid producing bacterial strain Pseudomonas aeruginosa M7⁃6, medium (carbon source,nitrogen source,C/N ratio,and mineral factors) was optimized for efficiently producing rhamnolipid under simulated oil reservoir conditions( temperature,pH and absence of oxygen) . Using the optimized medium,effect of different inoculums amount on anaerobic production of rhamnolipid by M7⁃6 was studied. Strain M7⁃6 was cultured in a 6⁃L anaerobic fermentor using oilfield production water to prepare medium,to evaluate the rhamnolipid production by M7⁃6 under simulated conditions and study the competitive activity of M7⁃6 with other native microorganisms. M7⁃6 could efficiently produce rhamnolipid under simulated conditions with glycerol as carbon source and nitrate as nitrogen source.The optimum C/N ratio is 14. 4∶1,and the least inoculums amount is 1%(V/V).Strain M7⁃6 can reduce surface tension of culture system to 38. 4 mN/m in the anaerobic fermenter.Moreover,the numbers of oil degrading bacteria and acid producing bacteria were increased, and the number of sulfate reducing bacteria was decreased.关键词
鼠李糖脂/模拟油藏条件/厌氧激活剂/微生物采油Key words
rhamnolipid/simulate d oil reservoir conditions/anaerobic activator/microbial enhanced oil recovery分类
生物科学引用本文复制引用
郭超,赵峰,郑甜甜,史荣久,韩斯琴,崔庆峰,张颖..鼠李糖脂产生菌M76在模拟油藏条件下的培养基优化[J].生物加工过程,2016,14(3):68-74,7.基金项目
国家高技术研究发展计划（863计划） （）
