生物加工过程2016，Vol.14Issue(3)：75-80,6.DOI:10.3969/j.issn.1672-3678.2016.03.014
铜绿假单胞菌XJ601产鼠李糖脂的优化培养及其稳定性
Effects of medium components on rhamnolipid production and its stability by Pseudomonas aeruginosa XJ601
摘要
Abstract
The present work aims to investigate the effects of different medium components on rhamnolipid production by anthrone colorimetry quantitative analysis with Pseudomonas aeruginosa strain XJ601,which was isolated from crude oil⁃contaminated sample. The results showed that water⁃soluble and water⁃insoluble carbon sources had been utilized forwere used to productione of rhamnolipids, while carbon sources such as vegetable oils were especially effective to produce rhamnolipid. With rapeseed oil as corbon source,nitrate was better than ammonium chloride as nitrogen source to enhance the production of rhamnolipids.Not only the type of carbon and nitrogen source but also the C/N ratio strongly influenced total rhamnolipid productivity,the highest final rhamnolipid concentration was observed at a C/N ratio of 20.The addition of P was also effective on the biosynthesis of rhamnolipids.The product of rhamnolipids by shake flask culture had good stability at different temperature and pH and different concentration of NaCl, which suggested that rhamnolipids produced by this strain hold much promise for oil recovery operations as well as for oil spill bioremediation.关键词
铜绿假单胞菌/鼠李糖脂/优化培养基/稳定性/定量分析Key words
Pseudomonas aeruginosa/rhamnolipid/optimization of medium components/stability/quantitative analysis分类
生物科学引用本文复制引用
罗娜,董文,何春秋,薛姝雯,李晶,陈富林,马艳玲..铜绿假单胞菌XJ601产鼠李糖脂的优化培养及其稳定性[J].生物加工过程,2016,14(3):75-80,6.基金项目
国家高技术研究发展计划（863计划）（2013AA064402）；陕西省教育厅产业化培育项目 （）
