生物技术通报2016，Vol.32Issue(5)：99-106,8.DOI:10.13560/j.cnki.biotech.bull.1985.2016.05.013
大口黑鲈北方亚种和佛罗里达亚种NPY基因的DNA和cDNA克隆及序列分析
Cloning and Analysis of DNA and cDNA Sequence in NPY Gene of Northern and Florida Subspecies of Largemouth Bass
摘要
Abstract
Neuropeptide Y(NPY)plays an important role in the activity of body feeding,and is the most potent endogenic orexigenic factor in mammals. In order to understand the structure and the function ofNPY gene in the largemouth bass(Micropterus salmoide),the NPY’s cDNA of northern and Florida subspecies of largemouth bass were cloned by RT-PCR and RACE techniques. The result revealed that theNPY’s cDNA in two subspecies all contained an ORF encoding 99 amino acids and 5′-UTR of 52 bp. Using PCR and genomewalker technology,a 3 561 bp and a 3 565 bp DNA sequence of of NPY gene were acquired from northern and Florida subspecies of largemouth bass respectively. Sequence analysis indicated that gene from both subspecies contained 4 exons and 3 introns. By bioinformatics analysis with MATINSPECTOR,the basic transcriptional regulatory elements such as TATA Box,CAAT Box,CCAAT Box,and GATA Box were found in the promoters of both subspecies. Six single-site base differences between thetwo genomic NPYDNA sequences of 2 subspecies were discovered,4-base insert segments were identified in promoter sequence of Florida subspecies compared with the northern one. The homology of NPY genes with other species was also compared,the largemouth bass shared the highest nucleotide homology with mandarin fish and grouper, reached 90% and 88% respectively,and the amino acid homology were 93% and 95%.关键词
大口黑鲈/亚种/食欲/NPY基因Key words
largemouth bass/subspecies/appetite/NPY gene引用本文复制引用
刘浩,白俊杰,李胜杰..大口黑鲈北方亚种和佛罗里达亚种NPY基因的DNA和cDNA克隆及序列分析[J].生物技术通报,2016,32(5):99-106,8.基金项目
国家科技支撑计划（2012BAD26B03），国家自然科学基金项目（31201985），“948”计划重点项目 （）
