生物技术通报2016，Vol.32Issue(5)：165-171,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016.05.022
产胞外多糖菌株的筛选及胞外多糖结构分析
Screening of Exopolysaccharide-producing Strains and Structural Analysis of the Exopolysaccharides
摘要
Abstract
This study aims to screen and identify strains producing exopolysaccharides(EPSs),to study the rheological properties of EPSs,and to analyze the structure of EPSs. The strains producing EPSs were screened from soil,and were identified based on their morphology and 16S rDNA gene sequencing. The viscosity changes of EPSs in different conditions were determined by viscometer to study its rheological properties. Then,GC,IR and NMR were used to analyze the structure of EPSs isolated and purified by ethanol precipitation,Sevag method and dialysis. As a result,a strain producing EPSs was identified asEnterobacter sp. WL113.The viscosity of the EPSs exponentially increased with the concentration of EPSs,i.e.,viscosity increased about 33 times when concentration of EPSs from 1 g/L to 10 g/L. The EPSs were sensitive to temperature,and viscosity decreased 89.8% when temperature from 25 ℃ to 100 ℃. The viscosity of EPSs remained relatively stable under the condition of acid,neutral and weak alkaline. The EPSs also well tolerated with metal ions such as Na+、K+、Mg2+ and Ca2+. The analysis of EPSs by GC,IR,and NMR indicated that the EPSs consisted of arabinose,mannose and glucose in a molar ratio of 1.63:3.16:2.38,also containingα-pyranose,β-pyranose and uronic acid.关键词
胞外多糖/流变学特性/肠杆菌/结构分析Key words
exopolysaccharide/rheological properties/Enterobacter/structural analysis引用本文复制引用
李彬,陈向楠,张建法,王世明..产胞外多糖菌株的筛选及胞外多糖结构分析[J].生物技术通报,2016,32(5):165-171,7.基金项目
中央高校基本科研业务费专项资金 （）
