生物技术通报2016，Vol.32Issue(5)：260-264,5.DOI:10.13560/j.cnki.biotech.bull.1985.2016.05.035
重组天花粉蛋白与YH-16联合用药抑制黑色素瘤细胞增殖及成瘤
The Inhibition Effect of Recombinant Trichosanthin Combined with YH-16 on the Proliferation and Xenograft Tumor Growth of Melanoma
郭会灿1
作者信息
- 1. 石家庄职业技术学院 化学工程系，石家庄 050081
- 折叠
摘要
Abstract
The objectives of this study are to acquire trichosanthin(rTCS)and to explore the effect of rTCS combined with YH-16 on the proliferation of melanoma B16-F10 and xenograft tumor growth in mice model. rTCS was expressed inEscherichia coliBL21 strain and purified by Ni affinity chromatography. Then the effects of rTCS,YH-16,and combination of rTCS with YH-16 on the cell growth were determined by MTT assay andin vivo trial. Results were as:pure recombinant rTCS protein was obtained. The proliferation of B16-F10 was inhibited significantly by rTCS,and inhibition rate was significantly higher than by YH-16 alone,however,no significant difference between rTCS alone and the combination of rTCS and YH-16. The xenograft tumor growth in the group treated with the combination of rTCS and YH-16 was significantly smaller than the ones by rTCS-treated alone or control. In conclusion,the combination of rTCS with YH-16 presented significant inhibitory effect on proliferation of B16-F10 as well as on xenograft tumor growth,and this strategy with the combination would provide new opportunity for treating melanoma patients.关键词
天花粉蛋白/YH-16/B16-F10/肿瘤/联合治疗Key words
trichosanthin/YH-16/B16-F10/tumor/combination therapy引用本文复制引用
郭会灿..重组天花粉蛋白与YH-16联合用药抑制黑色素瘤细胞增殖及成瘤[J].生物技术通报,2016,32(5):260-264,5.
