天津中医药2016，Vol.33Issue(5)：295-298,4.DOI:10.11656/j.issn.1672-1519.2016.05.10
健脾益肺化痰方通过抑制TNF-α信号通路改善COPD大鼠气道黏液高分泌的实验研究
Experimental study on Jianpi Yifei Huatan decoction improvement airway mucus hypersecretion in COPD rats through inhibition of TNF-α signaling pathway
摘要
Abstract
[Objective] To explore the mechanism of improvement of mucus hypersecretion in COPD rats by Jianpi Yifei Huatan decoction. [Methods] The rat model of COPD was established by tobacco smoke inhalation,and then the rats were randomly divided into the normal group,model group,Jianpi Yifei Huatan decoction group,Huatan decoction group, Jianpi Yifei Huatan decoction group and carbocisteine group. The normal group and model group were given normal saline solution intragastrically and other groups were given relevant medicines for 28 days,respectively. TNF-α in bronchoalveolar lavage fluid was detected by ELISA, and phospho-EGFR, phospho-PI3K and phospho-AKT in lung were detected by Western Blot. [Results] Compared with the normal group, the expressions of TNF-α, phospho-EGFR, phospho-PI3K and phospho-AKT in model group rats were significantly higher (P<0.01). Compared with model group,the expressions of TNF-α, phospho-EGFR, phospho-PI3K and phospho-AKT in all treating group rats were significantly lower (P<0.01 or P<0.05) or with a downward tendency. [Conclusion] Jianpi Yifei Huatan decoction improves airway mucus hypersecretion in COPD rats through inhibition of TNF-αsignaling pathway, and the role of "treating both symptoms and root causes"is better than single"treating symptoms"or"root causes".关键词
慢性阻塞性肺疾病/黏液高分泌/健脾益肺化痰方/肿瘤坏死因子-α/表皮生长因子受体Key words
chronic obstructive pulmonary disease/mucus hypersecretion/Jianpi Yifei Huatan decoction/TNF-α/EGFR分类
医药卫生引用本文复制引用
杨晓敏,赵娜妹,刘娟,邓秀兰,王青青,张晓晶,潘霏,李配,钟相根..健脾益肺化痰方通过抑制TNF-α信号通路改善COPD大鼠气道黏液高分泌的实验研究[J].天津中医药,2016,33(5):295-298,4.基金项目
国家自然科学基金资助项目(81173314,81473666) （81173314,81473666）
国家中医药管理局中医药重点学科基本科研课题项目(2013-ZDXKKF-03). （2013-ZDXKKF-03）
