天津中医药2016，Vol.33Issue(5)：307-310,4.DOI:10.11656/j.issn.1672-1519.2016.05.13
参竹精片协同戊巴比妥钠对小鼠睡眠及5-HT的影响
Effects of Shenzhujing tablets for sodium pentobarbital-induced hypnosis in mice
摘要
Abstract
[Objective] To observe the effects of Shenzhujing tablets on sodium pentobarbital-induced hypnosis. [Methods] The 96 of Kunming mice were randomly divided into 4 groups:normal group, and Shenzhujing tablet (0.5, 0.25, 0.125 g/kg) groups. The drugs were administrated orally in single doses to 12 of the mice in each group 2 h before the intraperitoneal injection of sodium pentobarbital at the dose of 30 mg/kg or 50 mg/kg, respectively. Then the mice received daily oral treatments of drugs for 7 days. Similarly, Sodium pentobarbital-induced hypnosis tests were also used 2 h after the last administration of the drugs. The sleep latency and sleep duration during pentobarbital-induced sleep were measured and the ratios of sleep mice to non-sleep mice were counted in mice after oral administration of the drugs. Moreover, the contents of the 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA)in the brain stem were determined. [Results] Compared with normal group, Shenzhujing tablets significantly prolonged the pentobarbital-induced sleep duration and decrease sleep latency at dose of 0.5 g/kg (P<0.05), increase the ratios of sleep mice (P<0.01) to non-sleep mice and the contents of 5-HT in the brain stem under the condition of 30 mg/kg of sodium pentobarbital (P<0.05). [Conclusion] Shenzhujing tablets possess sedative-hypnotic activity possibly by promoting the secretion of 5-HT in the brain and might be effective agents for treatment of insomnia.关键词
失眠/参竹精片/5-HTKey words
insomnia/Shenzhujing tablet/5-HT分类
医药卫生引用本文复制引用
赵宏杰,樊官伟,罗毅杨,高笑雨,齐记,柳占彪,郭利平..参竹精片协同戊巴比妥钠对小鼠睡眠及5-HT的影响[J].天津中医药,2016,33(5):307-310,4.基金项目
天津市现代中药重点实验室-省部共建国家重点实验室培育基地. （）
