中国组织工程研究2016，Vol.20Issue(19)：2796-2802,7.DOI:10.3969/j.issn.2095-4344.2016.19.009
瑞舒伐他汀联合脐血间充质干细胞移植改善急性心肌梗死后心功能
Rosuvastatin combined with umbilical cord blood mesenchymal stem cell transplantation improves cardiac function after acute myocardial infarction
王艳丽 1李金峰 1王艳萍 2高明1
作者信息
- 1. 唐山市开滦总医院心内科，河北省唐山市 063000
- 2. 唐山市遵化市人民医院，河北省唐山市 064200
- 折叠
摘要
Abstract
BACKGROUND:In recent years, it has been a hot topic that stem cel transplantation is used to improve cardiac insufficiency after acute myocardial infarction by inducing regeneration of cardiomyocytes in the infarction regions. OBJECTIVE:To observe the effect of rosuvastatin combined with umbilical cord blood mesenchymal stem cel s transplantation on rat cardiac function after acute myocardial infarction. METHODS:Forty-five Sprague-Dawley rats were enrol ed to prepare myocardial infarction models by ligaturing the left anterior descending coronary artery. Then they were equivalently divided into model group, transplantation group and combination group. At 7 days after modeling, rats in the combination group were given injection of 300μL umbilical cord blood mesenchymal stem cel s (15.0×108) via the tail vein and by gavage once a day for 28 days with 1 mg/kg rosuvastatin;rats in the transplantation group and model group were injected with 300μL umbilical cord blood mesenchymal stem cel suspension through the tail veins or the same amount of LG-DMEM medium, respectively, fol owed by intragastrical administration of the same amount normal saline. At 5 weeks after modeling, indexes of cardiac function, level of plasma Lp-PLA2 and heat shock protein 70 in the infarction regions were detected by color Doppler ultrasound, enzyme-linked immunosorbent assay and western blot assay, respectively. In addition, pathological changes of myocardial tissues were observed using hematoxylin-eosin staining. RESULTS AND CONCLUSION:Left ventricular ejection fraction and left ventricular end-systolic pressure were significantly higher in the combination group than in the transplantation group as wel as higher in the transplantation group than the model group (P<0.05);compared with the transplantation group, left ventricular end-diastolic pressure was significantly decreased in the combination group, but significantly increased in the model group (P<0.05);the number of cardiomyocytes in the infarction regions was significantly higher in the combination group than the other groups. Additional y, expression of heat shock protein 70 in the infarction regions was significantly increased in the combination group (P<0.05). To conclude, rosuvastatin combined with umbilical cord blood mesenchymal stem cel transplantation can significantly improve rat cardiac function after myocardial infarction.关键词
干细胞/移植/瑞舒伐他汀/脐血间充质干细胞/细胞移植/急性心肌梗死/心功能分类
医药卫生引用本文复制引用
王艳丽,李金峰,王艳萍,高明..瑞舒伐他汀联合脐血间充质干细胞移植改善急性心肌梗死后心功能[J].中国组织工程研究,2016,20(19):2796-2802,7.
