中国组织工程研究2016，Vol.20Issue(19)：2817-2823,7.DOI:10.3969/j.issn.2095-4344.2016.19.012
脐血间充质干细胞移植修复急性肠缺血再灌注损伤
Umbilical cord blood mesenchymal stem cell transplantation for acute intestinal ischemia-reperfusion injury
王炳杰 1胡延伟 2赵叶芳 3张仕东2
作者信息
- 1. 河北医科大学附属邢台市人民医院，肛肠外科，河北省邢台市 054001
- 2. 河北医科大学附属邢台市人民医院，普外科，河北省邢台市 054001
- 3. 河北医科大学附属邢台市人民医院，妇产科，河北省邢台市 054001
- 折叠
摘要
Abstract
BACKGROUND:Bone marrow mesenchymal stem cel s can repair intestinal ischemia-reperfusion injuny by interfering inflammatory reactions after intestinal ischemia-reperfusion to protect intestinal barrier functions. In recent years, umbilical cord blood mesenchymal stem cel s are gradual y used as a substitute source of bone marrow mesenchymal stem cel s. OBJECTIVE:To investigate the effects of umbilical cord blood mesenchymal stem cel s on acute intestinal ischemia-reperfusion injury. METHODS:Umbilical cord blood mesenchymal stem cel s were induced, isolated in vitro and tracked by CM-DiI fluorescent labeling. Sixty-three Sprague-Dawley rats were equivalently randomized into three groups:control group received normal saline enema, intestinal ischemia-reperfusion injury group with ethanol diluted trinitro-benzene-sulfonic acid and transplantation group administrated with 1×1010/L umbilical cord blood mesenchymal stem cel suspension via the tail vein at 1 hour after trinitro-benzene-sulfonic acid modeling. At 3 days after transplantation, colon tissues were removed in each group to observe pathological changes of the intestinal tract by hematoxylin-eosin staining. Besides, expression of leptin mRNA in the colon tissues and cyclooxygenase-2 in the mucosa were detected by RT-PCR and immunohistochemistry method, respectively. RESULTS AND CONCLUSION:Transplanted umbilical cord blood mesenchymal stem cel s distributed in the intestinal lymphoid tissue and among glandular epithelial cel s, suggesting that these stem cel s might be involved in the process of intestinal ischemia-reperfusion injury repair. Compared with the control group, intestinal injury in the injury group was significantly aggravated, and most intestinal epithelial cel s shed;and the transplantation group appeared to have significantly reduced intestinal damage and significantly less cel shedding. Expression of leptin mRNA was significantly higher in the injury group than the transplantation group fol owed by the control group, and there were significant differences among the three groups (P<0.05). Additional y, expression of cyclooxygenase-2 in the injury group was significantly higher than that in the control group (P<0.05);compared with the injury group, expression of cyclooxygenase-2 was significantly lower in the transplantation group (P<0.05). To conclude, leptin and cyclooxygenase-2 may be involved in acute intestinal ischemia-reperfusion injury, and umbilical cord blood mesenchymal stem cel transplantation significantly lessens intestinal ischemia-reperfusion injury, which provides an experimental basis for human treating acute intestinal ischemic injury.关键词
干细胞/移植/脐血间充质干细胞/大鼠/肠道损伤/缺血再灌注损伤分类
医药卫生引用本文复制引用
王炳杰,胡延伟,赵叶芳,张仕东..脐血间充质干细胞移植修复急性肠缺血再灌注损伤[J].中国组织工程研究,2016,20(19):2817-2823,7.
