中国组织工程研究2016，Vol.20Issue(20)：2927-2932,6.DOI:10.3969/j.issn.2095-4344.2016.20.006
脐血间充质干细胞移植联合紫杉醇对卵巢癌A2780细胞增殖、凋亡及侵袭的影响
Effects of combined treatment using umbilical cord blood mesenchymal stem cell transplantation and paclitaxel on proliferation, apoptosis, and invasion of A2780 ovarian cancer cells
陈逢振 1尹利荣1
作者信息
- 1. 天津医科大学第二医院妇科，天津市 120000
- 折叠
摘要
Abstract
BACKGROUND:An intensivestudy on ovarian cancer A2780 cels contributes to modify treatment strategies targeting ovarian cancer stem cels and enhance survival rate of ovarian cancer patients. <br> OBJECTIVE:To investigate the effects of umbilical cord blood mesenchymal stem cels (UC-MSCs) transplantation combined with paclitaxel on proliferation, apoptosis, and invasion of A2780 human ovarian cancer celsin vitro. <br> METHODS:A2780 human ovarian cancer cels were assigned into three groups: blank control group (routine cel culture),UC-MSCs group (addition of suspension containing 1×106UC-MSCs) and combined treatment group (combined addition of suspension containing 1×106UC-MSCs and 250μL of 1μmol/L paclitaxel solution). CD133+antigen expression, proliferation, apoptosis, and invasion were determined by immunofluorescent staining, flow cytometry, TUNEL staining, and Transwel chamber invasion assay, respectively. <br> RESULTS AND CONCLUSON:CD133+antigen expression intheUC-MSCs group and combined treatment group was significantly decreased compared withtheblank control group (P< 0.05). Proliferation and invasion abilities were significantly decreased, while apoptosis cel number was significantly increased inthecombined treatment group compared with the other two groups (P< 0.05). These findings indicate that the combined treatment of UC-MSCs transplantation and paclitaxel can inhibit proliferation and invasion and promote apoptosisof A2780 human ovarian cancer cels.关键词
组织构建/组织工程/脐血间充质干细胞/紫杉醇/卵巢癌/A2780 细胞/细胞增殖/细胞凋亡/细胞侵袭分类
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陈逢振,尹利荣..脐血间充质干细胞移植联合紫杉醇对卵巢癌A2780细胞增殖、凋亡及侵袭的影响[J].中国组织工程研究,2016,20(20):2927-2932,6.
