中国组织工程研究2016，Vol.20Issue(20)：2972-2978,7.DOI:10.3969/j.issn.2095-4344.2016.20.012
制备小鼠去细胞拟胚体对Lewis肺癌细胞的促分化作用
Acellular embryoid bodiesin mice：preparation and effect of promoting differentiationofLewis lung carcinoma cells
摘要
Abstract
BACKGROUND:Co-culture withembryonic stem cels or embryonic tissues can induce differentiation of carcinoma cels into normal epithelial cels or decreasemalignancyof carcinoma cels.Acelular embryoid bodies retain the structure and important cytokines of embryonic tissues. <br> OBJECTIVE:To prepare acelular embryoid bodies from mouse embryonic stem cels and to investigate their effects on differentiation of mouse Lewis lung carcinoma cels at three-dimensional culturein vitro. <br> METHODS:Mouse embryonic stem cels(D3)were dynamicaly cultured for 7 days to produce embryoid bodiesfolowedbydecelularization with 0.1% sodium dodecyl sulfate. Mouse Lewis lung carcinoma cels were co-cultured with acelular embryoid bodiesas test group or culturedinthree-dimensionalmatrigel mediumfor 7 days as control group, respectively. Cel proliferation and expression of E-cadherin were detected by immunohistochemical staining and western blot assay, respectively. In addition, mRNA expressions ofSlug and E-cadherin were observed using RT-PCR technology. <br> RESULTSAND CONCLUSION:Uniform mouse embryoid bodieswere successfuly prepared, andwere completely decelularized with sodium dodecyl sulfate. After 7-day three-dimensionalmatrigelculture, in the control group,multicelular tumor spheroidswere formed,accompanied byahigherKi67positive rate;Lewis lung carcinoma cels in the test group were repopulated in the acelular embryoid bodies showing significantly lowerKi67positive rate. Compared with the control group, the absorbance ofPaxilin in the test group was significantly smaler, and the absorbance of E-cadherin was significantly higher (P< 0.05). Besides, mRNA expressions of Slug and E-cadherin were significantly decreased and increasedin the test group compared with the control group, respectively(P< 0.05). These findings indicate that the acelular embryoid bodies can promote differentiation of mouse Lewis lung carcinoma celsinthree-dimensional culturein vitro.关键词
组织构建/组织工程/胚胎/去细胞/拟胚体/Lewis肺癌细胞/细胞外基质/生物材料/三维支架/三维培养/国家自然科学基金分类
医药卫生引用本文复制引用
吕卫东,蔡琳,张家东,雷光焰,刘志刚,张新伟,陆建荣..制备小鼠去细胞拟胚体对Lewis肺癌细胞的促分化作用[J].中国组织工程研究,2016,20(20):2972-2978,7.基金项目
国家自然科学基金资助项目(31100686)；陕西省自然科学基金面上项目(2015JM8435)@@@@the National Natural Science Foundation of China, No.31100686 （31100686）
the Natural Science Foundation of Shaanxi, No.2015JM8435 （）
