西南林业大学学报2016，Vol.36Issue(3)：1-6,6.DOI:10.11929/j.issn.2095-1914.2016.03.001
热激对多歧苏铁叶绿素含量及荧光特性的影响
Effects of Heat Shock on Chlorophyll Content and Fluorescence Characteristics of Cycas multipinnata
摘要
Abstract
In order to investigate the physiological responses of C. multipinnata to heat stress and explore the ecological adaptive mechanisms of this species under heat stress conditions. Detached leaves were used to compare the effects on chlorophyll content and fluorescence parameter of different temperatures ( 25, 35, 45, 55℃) . The results showed that the content of chlorophyll a, chlorophyll b and chlorophyll ( a+b) first rising then falling with the increase of temperature. They were decreased to the lowest level after heat shock at 45℃ and then increased af-ter heat shock at 55℃. However, chlorophyll a/b did not vary significantly among leaves treated at different tem-peratures. Fo, Fm, Fv/Fm, Y(NPQ), Y(NO), qP, NPQ, ETRmax,αand Ik did not vary significantly, but Y(Ⅱ) of leaves heated at 35℃ was significantly higher than that of leaves heated at 25-45℃. After leaves heated at 55℃, Ik did not change significantly, but Fo and Y( NO) increased significantly and other fluorescence parameters including Fm, Fv/Fm, qP, NPQ, ETRmax and α decreased significantly, compared with that of leaves heated at 25-45℃. The fraction of absorbed light allocated to antenna heat dissipation ( D) increased with the increase of temperature. The fraction of absorbed light in photochemistry ( P ) reached to the highest at 35 ℃ but then de-creased to the lowest at 55℃. The fraction of excess excitation energy ( E) did not vary significantly at 25-45℃, but decreased significantly at 55 ℃. In conclusion, C. multipinnata possessed relatively high tolerance to heat stress, and the photosynthetic activity was not affected at 25-45℃; however, the activity of PSⅡreaction center was decreased and the pathway of photosynthetic electron transport was inhibited at 55℃.关键词
多歧苏铁/热激/叶绿素含量/荧光特性/能量耗散Key words
Cycas multipinnata/heat shock/chlorophyll content/fluorescence characteristics/excitation en-ergy dissipation分类
农业科技引用本文复制引用
郑艳玲,马焕成..热激对多歧苏铁叶绿素含量及荧光特性的影响[J].西南林业大学学报,2016,36(3):1-6,6.基金项目
国家自然科学基金项目(31560093)资助；西南林业大学云南省省级重点学科(林学)资助；西南地区生物多样性保育国家林业局重点实验室开放基金资助。 （31560093）
