西南林业大学学报2016，Vol.36Issue(3)：7-11,5.DOI:10.11929/j.issn.2095-1914.2016.03.002
疑似多倍体小木漆的倍性鉴定
P loidy Identification of P seudo-P olyploidy in Toxicodendron vernicifluum
摘要
Abstract
With a pseudo-polyploidy of Toxicodendron vernicifluum in Zhaotong, Yunnan province as the ex-perimental materials, and the diploid T. vernicifluum as the control group, ploidy were determined by observation of the stomotal characteristic of leaf lower epidermis, analysis of flow cytometry and measurement of genome size. The results showed that the stomata density of pseudo-polyploidy of T. vernicifluum was 0. 52 times, the length of stomata was 1. 38 times, and the number of chloroplast in the guard cells was 1. 73 times compared with diploid T. vernicifluum, respectively. According to the results by flow cytometry analysis, the ploidy of somatic cell in pseu-do-polyploidy of T. vernicifluum was 1. 5 times to that of the diploid T. vernicifluum, the average genome sizes ( the content of 2C DNA) of pseudo-polyploidy of T. vernicifluum and diploid T. vernicifluum was about (1. 22 ± 0. 06) pg and (0. 96 ± 0. 04) pg, respectively, and the average genome sizes of pseudo-polyploidy of T. vernicifluum was 1. 27 times to diploid T. vernicifluum. Above all, it could be concluded that the pseudo-polyploidy of T. vernicifluum was a natural triploid lacquer.关键词
小木漆/倍性/基因组大小/流式细胞仪Key words
Toxicodendron vernicifluum/ploidy/genome size/flow cytometry分类
农业科技引用本文复制引用
韩国伟,李旦,赵月明,田斌,和润喜,欧光龙,尹五元,何承忠..疑似多倍体小木漆的倍性鉴定[J].西南林业大学学报,2016,36(3):7-11,5.基金项目
云南省教育厅重大专项( ZD2013014)资助；西南林业大学云南省省级重点学科(林学)建设项目资助；西南林业大学大学生创新基金项目(C1475)资助；西南林业大学科研启动基金项目(111409)资助；西南地区生物多样性保育国家林业局重点实验室开放基金资助。 （ ZD2013014）
