西南林业大学学报2016，Vol.36Issue(3)：80-85,6.DOI:10.11929/j.issn.2095-1914.2016.03.014
金铁锁离体快繁技术研究
The Research on in Vitro Rapid P ropagation of Psammosilene tunicoides
摘要
Abstract
In order to protect the wild resource of Psammosilene tunicoides effectively, tender stems of P. tunicoides were used as explants to study the in vitro rapid propagation technique of P. tunicoides. The results showed that the method of sterilization was that tender stems were rinsed with water, then disinfect with 75% alco-hol for 15 s and 0. 1% mercuric for 10 min. The contamination rate was 3. 61%. Differentiation culture medium was MS+2. 0 mg/L 6-BA+0. 05 mg/L TDZ+0. 05 mg/L NAA, the average quantity of differentiation bud was 3. 49, proliferation culture medium was MS+0. 3 mg/L 6-BA+0. 05 mg/L TDZ+0. 01 mg/L NAA, the proliferation mul-tiple was 4. 29, Rooting culture medium was 1/2MS+0. 3 mg/L IBA+0. 1 mg/L NAA+0. 3 g/L activated carbon, the rooting rate was 91. 7%, the tissue culture seedlings were transplanted into the matrix ( red soil ∶ humus ∶perlite=1∶1∶1) , the survival rate was 94. 5%. The technology of tissue culture is hopeful to solve the problem of wild resource shortage and insufficient supply of raw materials in the production.关键词
金铁锁/离体快繁/外植体/炼苗/培养基Key words
Psammosilene tunicoides/in vitro rapid propagation/explant/acclimatization/culture medium分类
农业科技引用本文复制引用
李斌,唐军荣,陈杰,尹丽莎,韩国伟,于鑫,辛培尧..金铁锁离体快繁技术研究[J].西南林业大学学报,2016,36(3):80-85,6.基金项目
西南林业大学云南省省级重点学科(林学)资助；云南省省院省校教育合作咨询共建重点学科项目( No.211015)资助；西南地区生物多样性保育国家林业局重点实验室开放基金资助。 （林学）
