烟草科技2016，Vol.49Issue(5)：16-22,7.DOI:10.16135/j.issn1002-0861.20160503
成熟期温度对烟叶质体色素积累及香气成分的影响
Effects of temperature on plastid pigment accumulation and aroma components in tobacco leaves at mature stage
摘要
Abstract
To study the effects of temperature on the quality and taste style of tobacco leaves at mature stage, a pot experiment using flue-cured cv. K326 (Nicotiana tabacum) was carried out. After topping, tobacco plants were transferred into artificial climate chambers with daily average temperature of 28.1 ℃ and 22.8 ℃, respectively. Cell structure, pigment accumulation and aroma components in middle leaves were analyzed after growing for 30 days. The leaf ultrastructure analysis indicated that under the higher temperature treatment (28.1 ℃) at mature stage, the cell nucleus in leaves degraded more completely, the content of starch grain was more abundant, the number and volume of osmiophilic particles increased, chloroplasts fully degraded and thylakoid lamellae partially completed. LC/MS analysis showed that the contents of plastid pigments, includingβ-carotene, neoxanthin, violaxanthin, lutein, chlorophyll a and chlorophyll b, under the higher temperature treatment were lower than those under the lower temperature treatment. However, the contents of carotenoids degradation products (including linalool, isophorone, β-damascone, dihydroactinidiolide, megastigmatrienone-4, farnesylacetone, solavetivone) and chlorophyll degradation product (neophytadiene) in leaves under the higher temperature treatment were significantly higher than those under the lower temperature treatment. In conclusion,the higher temperature at mature stage promotes the degradation of cell structure and plastid pigment and the accumulation of cell inclusion, which is beneficial to the generation of aroma substances and greatly improves the quality and taste style of tobacco leaves.关键词
烟叶/成熟期/温度/质体色素/香气物质Key words
Tobacco leaf/Mature stage/Temperature/Plastid pigment/Aroma substance分类
农业科技引用本文复制引用
杨永霞,张嘉炜,贾宏昉,路小改,杨胜男,连文力,崔红..成熟期温度对烟叶质体色素积累及香气成分的影响[J].烟草科技,2016,49(5):16-22,7.基金项目
中国烟草总公司重大专项“浓香型特色优质烟叶开发”（110200902045 TS-01）。 （）
