医药导报2016，Vol.35Issue(6)：583-587,5.DOI:10.3870/j.issn.1004-0781.2016.06.007
川芎嗪联合顺铂对Lewis肺癌中Arresten和整合素α1β1及VEGF表达的影响
Effect of Tetramethylpyrazine Combined with Cisplatin on Expression of Arresten, Integrin α1β1,VEGF of Lewis Lung Cancer Mice
朱亚芳 1张志华 2张志林 2张秀珑 2汤建华 3郑英娟 2张长洪2
作者信息
- 1. 河北北方学院研究生部，张家口 075000
- 2. 河北北方学院附属第一医院呼吸内科，张家口 075000
- 3. 河北北方学院附属第一医院药剂科，张家口 075000
- 折叠
摘要
Abstract
Objective To investigate the inhibition mechanism of tetramethylpyrazine combined with cisplatin on angiogenesis in Lewis lung cancer mice and to observe the mechanism of Arresten on angiogenesis in lung cancer. Methods The model of Lewis lung adenocarcinoma mouse xenograft was established in this work, and 40 mice were randomly divided into 4 groups: 0.9% sodium chloride solution group(NS group), tetramethylpyrazine group(TMP group), cisplatin group(DDP group), tetramethylpyrazine plus cisplatin group(TMP + DDP group), 10 mice in each group.Mice in NS group were given 0.2 mL of 0.9% sodium chloride solution, mice in DDP group were given 0.2 mL of 2 mg.kg-1 of cisplatin, mice in TMP group were given 0.2 mL of 100 mg.kg-1 of tetramethylpyrazine, mice in TMP+DDP group were given 2 mg.kg-1 of cisplatin and 100 mg.kg-1 of tetramethylpyrazine, each 0.1 mL .Tumor size was measured every day to calculate the tumor volume.The mice were sacrificed to stripp the subcutaneous tumor after continuous medication. The expressions of Arresten, integrin α1β1 and VEGF were determinated by immunhistochemistry and Western blotting. Results The tumor growth of NS group was the fastest and TMP+DDP group was the slowest. Compared with NS group, the expression of Arresten in the other three groups was increased( P<0.01) , and the TMP+DDP group exhibited the highest expression;at the same time, integrin α1β1 , VEGF in the other three groups was decreased(P<0.01), and the TMP+DDP group exhibited the lowest expression.The expression of integrinα1β1 and VEGF was negatively related to Arresten, and the expression of integrin α1β1 was positively correlated with VEGF. Conclusion TMP can inhibited the growth of Lewis lung carcinoma and angiogenesis. Moreover, in combination with cisplatin, TMP can also improved the effect of chemotherapy and then the survival state of mice. The mechanism of action, which TMP suppress tumor angiogenesis may be through improving Arresten and inhibiting integrin α1β1 and VEGF. And the action mechanism of Arresten may be implemented by inhibiting the expression of VEGF by incorporation with integrinα1β1 or by itself to inhibit the expression of VEGF.关键词
川芎嗪/顺铂/肺癌/血管内皮生长因子/整合素α1β1Key words
Tetramethylpyrazine/Cisplatin/Cancer, lung/Vascular endothelial growth factor/Integrin α1β1分类
医药卫生引用本文复制引用
朱亚芳,张志华,张志林,张秀珑,汤建华,郑英娟,张长洪..川芎嗪联合顺铂对Lewis肺癌中Arresten和整合素α1β1及VEGF表达的影响[J].医药导报,2016,35(6):583-587,5.
