中国肺癌杂志2016，Vol.19Issue(5)：247-251,5.DOI:10.3779/j.issn.1009-3419.2016.05.11
系统性鉴定长非编码RNA MALAT1调控的微小RNA
Comprehensive Identification of MicroRNAs Regulated by Long Non-coding RNA MALAT1
摘要
Abstract
Background and objective Long non-coding RNA (lncRNA) plays important regulatory roles in the development and invasion of various cancers. hTe aim of current study is to comprehensively identify microRNAs (miRNA) regulated by lncRNA MALAT1 via experimental and bioinformatics methods.Methods Antisense oligonucleotides (ASO) speciifcally targeting MALAT1 were designed and synthesized. Atfer knockdown of MALAT1 by ASO in A549 cells, miRNA expression changes were proifled by TqaMan Low Density Array (TLDA). Gene set enrichment analysis (GSEA) was used to search enriched miRNAs among differentially expressed genes atfer knockdown of MALAT1.ResultsAtfer effcient knock-down of MALAT1 by ASO, 153 miRNAs were differentially expressed, 131 up-regulated and 22 down-regulated. Among the 458 differentially expressed genes atfer MALAT1 silence, GSEA results revealed lots of enriched miRNAs. hTere were 28 over-lapped miRNAs between TLDA and GSEA results, suggesting these 28 miRNAs are regulated by MALAT1.Conclusion hTis study comprehensively identiifed MALAT1 regulated miRNAs, providing resources for further research.关键词
长非编码RNA/MALAT1/microRNA/生物信息学Key words
Long non-coding RNA/MALAT1/MicroRNAs/Bioinformatics引用本文复制引用
张海天,王国祥,尹荣,邱满堂,许林..系统性鉴定长非编码RNA MALAT1调控的微小RNA[J].中国肺癌杂志,2016,19(5):247-251,5.基金项目
本研究受国家自然科学基金（No.81372321, No.81201830, No.81472200）、江苏省高校自然科学基金（No.13KJB320010）和江苏省重大疾病生物资源样本库（No.BM2015004）项目资助@@@@This study was supported by the grants from National Natural Science Foundation of China (to Lin XU)(No.81372321)(to Rong YIN)(No.81201830 and No.81472200), Natural Science Foundation for High Education of Jiangsu Province (to Rong YIN)(No.13KJB320010), and Jiangsu Province BioBank of Major Diseases (to Lin XU)(No.BM2015004) （to Lin XU）
