中国免疫学杂志2016，Vol.32Issue(6)：787-793,7.DOI:10.3969/j.issn.1000-484X.2016.06.004
滤泡辅助性T细胞在实验性自身免疫性脑脊髓炎发病过程中的作用
Mechanism of T follicular cells in experimental autoimmune encephalomyelitis
摘要
Abstract
Objective: To evaluate the mechanism of T follicular helper cells ( Tfh ) in experimental autoimmune encephalomyelitis (EAE) via in vivo experiments. Methods:C57BL/6 mice were randomly divided into four groups,CFA group,EAE group,anti-ICOSL group and control group. Lymphocytes of different time points isolated from draining lymph nodes and spleen were stained for T follicular helper cells surface marker and T cells activation surface marker and analyzed by FACS. Observed parameters include inflammatory infiltration,demyelination in spinal cord and germinal center in spleen. ELISA was used to measure the level of antigen specific antibodies. Results: Mice in anti-ICOSL treated group developed mild disease was with lower clinical scores when compared with the EAE group. HE staining results turned out with alleviated inflammation and Luxol Fast Blue staining( LFB) showed no demyelization in anti-ICOSL treated mice compared with non-treated EAE models. Flow cytometry results revealed that percentages of T follicular helper cells decreased though the whole activated degree T cells was not influenced in anti-ICOSL treated group. Fewer ger minal center was found in both anti-ICOSL group and CFA group with reduced secretion of MOG-specific Ab. Conclusion:T follicular helper cells supported the development of cognate B cells,promoted the formation of germinal center,facilitate pathogenic MOG-specific Ab secretion,thus enhance EAE.关键词
滤泡辅助性T细胞/实验性自身免疫性脑脊髓炎/生发中心Key words
T follicular helper cells( Tfh)/Experimental autoimmune encephalomyelitis( EAE)/Germinal center( GC)分类
生物科学引用本文复制引用
平昌昀,李呼伦,詹晓霞,孙博..滤泡辅助性T细胞在实验性自身免疫性脑脊髓炎发病过程中的作用[J].中国免疫学杂志,2016,32(6):787-793,7.基金项目
本文受国家自然科学基金（81571168 （）
81430035)资助。 （）
