中国免疫学杂志2016，Vol.32Issue(6)：828-831,4.DOI:10.3969/j.issn.1000-484X.2016.06.013
沉默GRP94基因对乳腺癌MCF7细胞增殖和凋亡的影响
Effect of GRP94 silencing on proliferation and apoptosis of human breast carcinoma MCF7 cells
樊建军 1武家燕 1李韵 1曾帆 1宋方洲1
作者信息
- 1. 重庆医科大学分子医学与肿瘤研究中心，生物化学与分子生物学教研室，重庆 400016
- 折叠
摘要
Abstract
Objective:To determine the effects of silencing glucose regulated protein ( GRP94 ) on the proliferation and apoptosis of breast carcinoma MCF7 cells. Methods:Chemically synthesized siRNA targeting GRP94 gene and transfected into MCF7 cells used by Liopfectamine RNAIMAX. The mRNA and protein expression levels of GRP94,cyclinD1,Bax and Bcl-2 were detected by Real-time PCR and Western blot. CCK8 assay was used to detect the effect of specific GRP94 siRNA on cell proliferation and the effect on cell cycle and apoptosis were analyzed by flow cytometry and Hoechst 33258 staining. Results:Compared with the siRNA-NC cells, the expression of GRP94 was significantly down-regulated in MCF7 cells. Knockdown of GRP94 in MCF7 cells decreased cell proliferation and promoted cell apoptosis. The expression of cyclinD1and Bcl-2levels were significantly reduced, and Bax level was increased in siRNA-GRP94 MCF7 cells. Conclusion: The siRNA-mediated GRP94 silence significantly inhibits MCF7 cell proliferation,promoted cell apoptosis by down-regulating cyclin D1,Bcl-2 expression and up-regulating the Bax expression in MCF7 cells.关键词
GRP94基因/MCF7细胞/增殖/凋亡Key words
GRP94 gene/MCF7 cells/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
樊建军,武家燕,李韵,曾帆,宋方洲..沉默GRP94基因对乳腺癌MCF7细胞增殖和凋亡的影响[J].中国免疫学杂志,2016,32(6):828-831,4.
