中国水产科学2016，Vol.23Issue(3)：584-593,10.DOI:10.3724/SP.J.1118.2016.15222
斜带石斑鱼幼鱼的饲料精氨酸需求量
Requirement of dietary arginine for juvenile orange-spotted grouper, Epinephelus coioides
摘要
Abstract
Seven isonitrogenous and isolipidic commercial diets with arginine contents of 2.13%, 2.42%, 2.71%, 2.95%, 3.20%, 3.48%, or 3.74%(dry matter) were formulated to investigate the effects of dietary arginine on growth performance, feed utilization, and serum total nitric oxide synthase in the juvenile orange-spotted grouper, Epinephelus coioides. Triplicate groups of 30 fish with mean body weight of (7.52±0.02) g were randomly as-signed to each treatment in separate tanks. The fish were fed the experimental diets for 8 weeks. The different levels of dietary arginine had no significant effect on the fish survival rate (P>0.05). However, the weight gain rate and specific growth rate were significantly higher in the 2.95% arginine group than in the other groups (P<0.05). The feed coefficient of the fish fed 2.95% arginine was significantly lower than that of the fish fed 2.13%, 2.42%, or 2.71% arginine (P<0.05). The whole-body protein of the fish fed 3.20% arginine was signifi-cantly higher than that of the fish fed 2.13% arginine (P<0.05). Serum glucose tended to decrease as dietary ar-ginine increased, and was significantly higher in the fish fed 2.13% or 2.42% arginine than in those of the other groups (P<0.05). Total protein and total nitric oxide synthase were significantly higher in the sera of fish fed 2.95% arginine than those in the sera of fish in the other groups (P<0.05). These results indicate that dietary ar-ginine not only promotes growth and body protein synthesis, but also improves the immunity of the grouper. A quadratic regression analysis of the specific growth rate against dietary arginine indicated that the optimum argin-ine level for the maximum growth of the juvenile grouper is 3.06%, corresponding to 6.07% of dietary protein on a dry weight basis.关键词
斜带石斑鱼/精氨酸/生长性能/生化指标Key words
Epinephelus coioides/arginine/growth performance/biochemical parameters分类
农业科技引用本文复制引用
韩凤禄,张琴,黄国强,迟淑艳,谭北平,董晓慧,杨奇慧,刘泓宇,章双..斜带石斑鱼幼鱼的饲料精氨酸需求量[J].中国水产科学,2016,23(3):584-593,10.基金项目
国家公益性行业(农业)专项资金项目(201003020) （农业）
广东省科技创新项目(2013KJCX0097) （2013KJCX0097）
广西海洋生物技术重点实验室开放基金资助课题(GLMBT-201405) （GLMBT-201405）
广东海洋大学优秀学位论文培育项目(校研[2014]3号) （校研[2014]3号）
