中国水稻科学2016，Vol.30Issue(3)：223-231,9.DOI:10.16819/j.1001-7216.2016.5177
水稻泛素结合酶基因家族的生物信息学与表达分析
Bioinformatic and Expression Analysis of Rice Ubiquitin-conjugating Enzyme Gene Family
摘要
Abstract
The ubiquitin /proteasome system plays an important role in plant growth and development ,morphogenesis and disease resistance .Recent studies have shown that some pathogens can mimic the host plant ubiquitin /proteasome system components to achieve their own purposes .Ubiquitin‐conjugating enzyme is the second enzyme in the ubiquitination process and is indispensable for the plant ubiquitin/proteasome system .Previous studies showed that there are 48 predicted ubiquitin‐conjugating enzyme genes in rice genome .In order to preliminarily elucidate the functions of rice ubiquitin‐conjugating enzyme genes in plant disease resistance ,bioinformatic ,RNA‐seq and qRT‐PCR methods were used to analyze characteristics and expression patterns of rice ubiquitin‐conjugating enzyme gene family . Phylogenetic tree analyses indicate that the 48 rice ubiquitin‐conjugating enzyme genes can be divided into 3 groups ,7 sub‐groups in total .Protein domain analysis showed that ubiquitin‐conjugating enzyme genes mainly consist of a big ubiquitin‐conjugating enzyme catalytic domain .Expression analysis in silico suggested that most of the rice ubiquitin‐conjugating enzymes can be induced by blast fungus infection .Plant cis‐acting elements analysis indicated that four pathogen resistance cis‐acting elements and one hypersensitivity reaction cis‐acting element have high distribution in the promoter region of the 48 rice ubiquitin‐conjugating enzyme genes .RNA‐seq data from compatible and incompatible monogenic rice after rice blast fungus infection showed that 44 rice ubiquitin‐conjugating enzyme genes were expressed at 36 hours after treatment ,among which more than 50% were highly expressed genes .qRT‐PCR analysis showed that expression of some ubiquitin‐conjugating enzyme genes can be induced by the inoculation of rice blast fungus both in compatible and incompatible monogenic rice .However ,in incompatible rice the expression of rice ubiquitin‐conjugating enzyme genes tends to be inhibited after rice blast fungus inoculation .关键词
水稻/泛素结合酶/泛素/蛋白酶体系统/生物信息学/稻瘟病菌/RNA-seq/qRT-PCRKey words
rice/ubiquitin-conjugating enzyme/ubiquitin/proteasome system/bioinformatics/Magnaporthe oryzae/RNA-seq/qRT-PCR分类
生物科学引用本文复制引用
刘鑫,张恒,阚虎飞,周立帅,黄昊,宋林林,翟焕趁,张君,鲁国东..水稻泛素结合酶基因家族的生物信息学与表达分析[J].中国水稻科学,2016,30(3):223-231,9.基金项目
国家科技支撑计划资助项目（2013BAD19B03）。 （）
