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水稻迟抽穗突变体 dth9的遗传分析与基因定位

叶卫军 胡时开 吴立文 郭龙彪 钱前

中国水稻科学2016,Vol.30Issue(3):232-238,7.
中国水稻科学2016,Vol.30Issue(3):232-238,7.DOI:10.16819/j.1001-7216.2016.5156

水稻迟抽穗突变体 dth9的遗传分析与基因定位

Genetic Analysis and Gene Mapping of a Heading-delayed Mutant dth9 in Rice (Oryza sativa L .)

叶卫军 1胡时开 2吴立文 3郭龙彪 4钱前3

作者信息

  • 1. 浙江大学 农业与生物技术学院,杭州 310058
  • 2. 中国水稻研究所,杭州 310006
  • 3. 中国水稻研究所,杭州 310006
  • 4. 中国农业科学院 深圳农业基因组研究所,广东深圳 518120
  • 折叠

摘要

Abstract

A heading‐delayed mutant ,dth9 (days to heading 9)was identified from an ethyl methylsulfonate (EMS)‐induced 93‐11 mutant library .The heading date of dth9 delayed about 50 days compared to the wild‐type and there was no significant difference in other agronomic traits .Genetic analysis showed that the phenotype of dth9 was controlled by a single recessive nuclear gene .To map this gene ,two F2 populations were generated by crossing the dth9 mutant with Nipponbare or Wuyunjing 7 as mapping populations .By using SSR markers and eight new designed InDel markers , DT H9 was narrowed to a 240kb interval between the markers D9‐9 and D9‐17 near the centromere of chromosome 9 , there were no reports about genes associated with heading date in this interval .In addition ,the expression levels of genes related to heading date were significantly decreased in dth9 by quantitative real‐time PCR analysis .

关键词

水稻/抽穗期/遗传分析/基因定位

Key words

rice/heading date/genetic analysis/gene mapping

分类

生物科学

引用本文复制引用

叶卫军,胡时开,吴立文,郭龙彪,钱前..水稻迟抽穗突变体 dth9的遗传分析与基因定位[J].中国水稻科学,2016,30(3):232-238,7.

基金项目

国家自然科学基金资助项目(31501279,31521064,31271700);国家973计划资助项目(2013CBA01405);中国博士后科学基金资助项目(2015M570181);深圳市科技计划资助项目(JCYJ20150630165133402)。 ()

中国水稻科学

OA北大核心CSCDCSTPCD

1001-7216

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