中国水稻科学2016，Vol.30Issue(3)：239-246,8.DOI:10.16819/j.1001-7216.2016.5176
水稻类病斑突变体wy3的鉴定和基因定位
Characterization and Gene Mapping of Lesion Mimic Mutant w y3 in Rice
摘要
Abstract
A lesion mimic mutant wy3 was obtained from the progeny of a japonica variety Wuyujing 3 by natural mutation .The lesions were first observed on the leaves at the seedling stage ,then spread gradually to the whole leaf at the initial tillering stage .Compared with the wild type (WT ) ,the plant height ,the number of effective tiller ,panicle length ,grain number per panicle and seed setting rate of wy3 were reduced significantly .The shading assay showed that the lesions on leaves of w y3 were induced by natural light .Compared with the wild type , the photosynthetic pigment and the net photosynthetic rate of wy3 were significantly reduced ,but the SOD activity ,POD activity ,CAT activity and MDA content of wy3 were significantly higher than those of WT .Trypan blue staining experiments showed that there were a large number of dead cells in the mutant lesion .Genetic analysis suggested that the phenotype of wy3 was controlled by a single recessive nuclear locus ,and the target gene was mapped between markers W2‐17 and W2‐18 with the physical distance of 28 kb on chromosome 2 .Sequence analysis revealed that the mutated gene in w y3 had a single nucleotide deletion at the 375th in CDS of LOC_Os02g02000 ,resulting in a premature termination of translation of the target gene ,which is an new allele of OsH PL3 .关键词
水稻/类病斑突变体/基因定位Key words
rice/lesion mimic mutant/gene mapping分类
生物科学引用本文复制引用
张宏根,王茂宇,张丽佳,胡雅,马佳琦,张翼帆,汤述翥,梁国华,顾铭洪..水稻类病斑突变体wy3的鉴定和基因定位[J].中国水稻科学,2016,30(3):239-246,8.基金项目
国家自然科学基金资助项目（31071384，31000533）；江苏省科技支撑计划资助项目（BE2013301）；江苏高校优势学科建设工程资助项目。 （）
