中国水稻科学2016，Vol.30Issue(3)：256-264,9.DOI:10.16819/j.1001-7216.2016.5190
转Cry 30 Fa1基因抗褐飞虱水稻的获得及鉴定
Development and Identification of Insect Resistant Transgenic Rice with Cry30Fa1 Gene
摘要
Abstract
Modified Cry30Fa1 ,a Bacillus thuringiensis (Bt) gene encoding a high‐efficiency insecticidal protein to the brown planthopper(BPH) ,was introduced into Shuhui 818(R818) by Agrobacterium‐mediated genetic transformation and 46 transgene lines were obtained .We confirmed the valid transformation by RT‐qRCR and Western blot at transcriptional level and translational level ,and fixed the stable expression Cry30Fa1 in homozygosis .The classical pedigree breeding methods were also used to select the important agronomic traits in transgenic lines .Furthermore ,we evaluated the resistance to BPH of different lines in growth chamber and field ,and found that the R818‐Cry30Fal displayed higher resistance than untransformed R818 in seedling stage .We also observed the reduced population of BPH feeding the transgenic lines ,suggesting that the transformed Cry30Fa1 enhanced resistibility to BPH in rice .We cultivated new BT protein transgenic rice restorer line R818‐Cry30Fal ,which may provide new BPH resistance material to three line hybrid rice breeding and enrich BPH resistance germplasm resources in rice .关键词
褐飞虱/农杆菌介导法/抗虫性/Cry30Fa1Key words
brown planthopper/A grobacterium-mediated method/insect resistance/Cry30Fa1分类
生物科学引用本文复制引用
王海鹏,李双成,王玲霞,李平,王世全,黄晓西,梁越洋,朱军,张翠霞,王秀梅,贡常委,郑爱萍,邓其明..转Cry 30 Fa1基因抗褐飞虱水稻的获得及鉴定[J].中国水稻科学,2016,30(3):256-264,9.基金项目
国家转基因生物新品种培育科技重大专项（2011ZX08001001）。 （）
