中国水稻科学2016，Vol.30Issue(3)：304-312,9.DOI:10.16819/j.1001-7216.2016.5150
粳稻超亲变异系籽粒谷氨酰胺合成酶基因表达特性及序列变异分析
Expression Characteristics and Sequence Variation Analysis of Glutamine Synthetase Gene in Grain of japonica Rice with Transgressive Variation
摘要
Abstract
The transgressive variants derived from rice varieties with significant difference in grain protein content were used to investigate the expression level and structure of glutamine synthetase gene (GS ) , activity of glutamine synthetase and protein accumulation characteristics during grain filling .The results showed that transgressive variants could be obtained through successive directive selection of grain protein content ,because the grain protein content was closely related to corresponding genotype . During grain filling , the activity of glutamine synthetase followed a single‐peak curve ,highly correlated with grain protein content and significantly differed between parents and progenies . GS1 .3 and GS2 genes ,in transgressive variants and their parents with different protein content ,exhibited similar transcription trend during grain filling ,that is ,the transcription level increased 15 -20 days before heading and then decreased gradually .Moreover ,the grain protein content was closely related to GS1 .3 and GS2 expression levels .The varieties with higher GS transcription level showed higher protein content compared with their parents .In addition , although GS1 .3 and GS2 gene sequences showed high conservation ,the gene sequence and protein sequence of GS1 .3 and GS2 were not completely identical in different varieties ,and there are some single nuclear polymorphisms .Random base variation as well as changes in codon and amino acids might occur because inter‐variety sexual hybridization causes base substitution during segregation and stability .关键词
粳稻/超亲变异系/籽粒/GS基因表达/序列变异Key words
j aponica rice/transgressive variant/grain/GS gene expression/sequence variation分类
生物科学引用本文复制引用
徐振华,曲莹,刘海英,朱立楠,张忠臣,金正勋..粳稻超亲变异系籽粒谷氨酰胺合成酶基因表达特性及序列变异分析[J].中国水稻科学,2016,30(3):304-312,9.基金项目
科技部科技支撑计划资助项目（2015BAD23B05）；东北农业大学学科团队建设项目。 （）
