中国药房2016，Vol.27Issue(15)：2109-2110,2111,3.DOI:10.6039/j.issn.1001-0408.2016.15.31
HPLC法测定复方依沙吖啶软膏中乳酸依沙吖啶的含量
Content Determination of Ethacridine Lactate in Compound Ethacridine Ointment by HPLC
摘要
Abstract
OBJETCTIVE:To establish a method for the content determination of ethacridine lactate in Compound ethacridine ointment. METHODS:HPLC was performed on the column of Agilent ZORBAX SB-C18 with mobile phase of 0.1% Octanesulfon-ic acid sodium solution-acetonitrile(70∶30,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 270 nm,the column temperature was 30℃,and the injection volume was 10 μl. RESULTS:The linear range of ethacridine lactate was 10.002-50.010μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were less than 1%;recovery was 98.96%-100.36%(RSD=0.49%,n=9). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for content determina-tion of ethacridine lactate in Compound ethacridine ointment.关键词
复方依沙吖啶软膏/乳酸依沙吖啶/含量测定/高效液相色谱法Key words
Compound ethacridine ointment/Ethacridine lactate/Content determination/HPLC分类
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刘俊丽,田佳懿,段松冷,曾蔚欣,金锐,孙路路..HPLC法测定复方依沙吖啶软膏中乳酸依沙吖啶的含量[J].中国药房,2016,27(15):2109-2110,2111,3.基金项目
首都卫生发展科研专项项目 （）
