中国药房2016，Vol.27Issue(15)：2114-2116,3.DOI:10.6039/j.issn.1001-0408.2016.15.33
活血散的质量标准研究
Study on the Quality Standard of Huoxuesan
摘要
Abstract
OBJECTIVE:To establish the quality standard of Huoxuesan. METHODS:Microscopic identification method was adopted for the qualitative identification of Rhizoma pinelliae,Eupolyphaga seu,Phellodendron amurense and Fructus kochiae in the preparation. HPLC was adopted for the contents determination of phellodendrine,polydatin,jatrorrhizine,berberine and emo-din:the column was ZORBAX Eclipse Plus C18 with methanol-0.1% phosphoric acid(gradient elution)at a flow rate of 0.4 ml/min,the detection wavelength was 240 nm,column temperature was 30 ℃,and the injection volume was 5 μl. RESULTS:Micro-scopic identification map of R. pinelliae,E. seu,P. amurense and F. kochiae was clear. The linear range was 0.159-5.073 μg(r=0.997 4)for phellodendrine、0.149-4.761 μg(r=0.999 9)for polydatin、0.239-7.649 μg(r=0.995 5)for jatrorrhizine、0.165-5.268 μg (r=0.997 2)for berberine、0.012-0.382 μg(r=0.999 9)for emodin;RSDs of precision,stability and reproducibility tests were low-er than 3.0%;recoveries were 98.9%-104.1%(RSD=1.9%,n=6),96.1%-101.7%(RSD=2.5%,n=6),99.6%-105.1%(RSD=2.6%,n=6),90.3%-98.2%(RSD=2.9%,n=6)and 92.9%-96.4%(RSD=2.0%,n=6)respectively. CONCLUSIONS:The es-tablished standard can be used for the quality control of Huoxuesan.关键词
活血散/显微鉴别/含量测定/黄柏碱/虎杖苷/药根碱/小檗碱/大黄素Key words
Huoxuesan/Microscopic identification/Content determination/Phellodendrine/Polydatin/Jatrorrhizine/Berber-ine/Emodin分类
医药卫生引用本文复制引用
赵颖,周芙琼,朱维娜,李文,谈瑄忠..活血散的质量标准研究[J].中国药房,2016,27(15):2114-2116,3.基金项目
南京市医学科技发展项目 （）
