中国药房2016，Vol.27Issue(15)：2119-2121,3.DOI:10.6039/j.issn.1001-0408.2016.15.35
HPLC法同时测定复方麝香草酚溶液中3种有效成分的含量
Simultaneous Determination of 3 Effective Ingredients in Compound Thymol Solution by HPLC
陈伟 1杨珺 1杨谨成 1周海燕 1王宇 1李国辉1
作者信息
- 1. 中国医学科学院肿瘤医院药剂科，北京 100021
- 折叠
摘要
Abstract
OBJECTIVE:To establish amethod for simultaneous determination ofbenzoic acid,methylis salicylas and thymol in Compound thymol solution.METHODS:HPLC was performed on the column of Thermo Hypersil BDS C18 with the mobile phase ofacetonitrile-water-acetic acid(40∶60∶0.02,V/V/V)at a flow rate of 1 ml/min,the detection wavelength was 275 nm,column tem-perature was 30 ℃,and injection volume was 10 μl. RESULTS:The linear range was 0.045 2-0.9030 mg/ml for benzoic acid, 0.0492-0.9844 mg/ml for methylis salicylas and 0.046 4-0.927 0 mg/ml for thymol;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.40%-108.88%(RSD=3.26%,n=9),98.21%-104.07%(RSD=1.87%,n=9) and 96.83-107.09%(RSD=3.33%,n=9),respectively. CONCLUSIONS:The method is simple,accurate and specific,and accurately de-termine the contents of benzoic acid,methylis salicylas and thymol in Compound thymol solution.关键词
苯甲酸/水杨酸甲酯/麝香草酚/高效液相色谱法/含量测定Key words
Benzoic acid/Methylis salicylas/Thymol/HPLC/Content determination分类
医药卫生引用本文复制引用
陈伟,杨珺,杨谨成,周海燕,王宇,李国辉..HPLC法同时测定复方麝香草酚溶液中3种有效成分的含量[J].中国药房,2016,27(15):2119-2121,3.
