中国药房2016，Vol.27Issue(15)：2122-2125,2126,5.DOI:10.6039/j.issn.1001-0408.2016.15.36
LC-MS/MS法测定桑叶药材中100种农药残留量
Determination of 100 Pesticide Residues in Moras alba by LC-MS/MS
黄莉莉 1李丽莉 1罗轶 1陆敏仪 1黄清泉1
作者信息
- 1. 广西壮族自治区食品药品检验所，南宁 530021
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for the determination of 100 pesticide residues in Morus alba. METHODS:LC-MS/MS was conducted on the column of ZORBAX Eclipse plus C18 with mobile phase of acetonitrile (containing 5% water, 0.1% formic acid,5 mmol/L ammonium formate)- water(containing 0.1% formic acid,5 mmol/L ammonium formate)(gradient elution)at a flow rate of 0.4 ml/min,column temperature was 40 ℃,injection volume was 5 μl;MS conditions:ionization source was electrospray ionization with positive ion mode,scanning mode was dynamic MRM,scanning time window was 2 min;atomiz-ing gas was nitrogen,atomizing gas pressure was 40 psi,drying gas flow rate was 5 L/min,capillary voltage was 4 000 V,and ion spray voltage was 500 V. RESULTS:The linear range of 100 pesticide residues was 2.4-150 ng/ml(r>0.990 0),recovery was 69.3%-128.2%,and the determination limit was 0.003-16 μg/kg. CONCLUSIONS:The method is simple,stable and reproduc-ible,and can be used for the determination of pesticide residues in M. alba.关键词
农药/桑叶/液相色谱-串联质谱法Key words
Pesticide/Morus alba/LC-MS/MS分类
医药卫生引用本文复制引用
黄莉莉,李丽莉,罗轶,陆敏仪,黄清泉..LC-MS/MS法测定桑叶药材中100种农药残留量[J].中国药房,2016,27(15):2122-2125,2126,5.
