中国药房2016，Vol.27Issue(15)：2138-2140,3.DOI:10.6039/j.issn.1001-0408.2016.15.41
HPLC法同时测定复方消痔栓中3种成分的含量
Simultaneous Determination of Three Kinds of Components in Compound Xiaozhi Suppository by HPLC
齐广才 1吴江瑞 1张咪咪 1崔生飞 1刘珍叶1
作者信息
- 1. 延安大学化学与化工学院，陕西延安 716000
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of gallic acid,rhein and emodin in Com-pound xiaozhi suppository. METHODS:HPLC was performed on the column of Kromasil C18 with mobile phase of 0.5% phosphor-ic acid-Acetonitrile solution(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 273 nm for gallic acid and 254 nm for rhein and emodin,the column temperature was 28 ℃,and the injection volume was 20 μl. RESULTS:The linear range was 0.296 4-14.82 µg/ml for gallic acid(r=0.999 6),0.215 0-10.75 µg/ml for rhein(r=0.999 9)and 0.307 2-15.36 µg/ml for emo-din(r=0.999 9);RSDs of precision,stability and reroducibility tests were lower than 3.0%;recoveries were 95.1%-97.2%(RSD=0.64%,n=6),95.4%-97.2%(RSD=0.42%,n=6) and 96.5%-99.4%(RSD=1.10%,n=6),respectively. CONCLU-SIONS:The method is simple and accurate,and can be used for the contents determination of gallic acid,rhein and emodin in Compound xiaozhi suppository.关键词
复方消痔栓/没食子酸/大黄酸/大黄素/高效液相色谱法Key words
Compound xiaozhi suppository/Gallic acid/Rhein/Emodin/HPLC分类
医药卫生引用本文复制引用
齐广才,吴江瑞,张咪咪,崔生飞,刘珍叶..HPLC法同时测定复方消痔栓中3种成分的含量[J].中国药房,2016,27(15):2138-2140,3.
