中国药房2016，Vol.27Issue(16)：2225-2228,4.DOI:10.6039/j.issn.1001-0408.2016.16.19
雷帕霉素对宫颈癌HeLa细胞侵袭转移的影响及机制研究
Study on Effects and Mechanism of Rapamycin on Invasion and Metastasis of Cervical Cancer HeLa Cells
贾利刚 1田菲 1张媛1
作者信息
- 1. 河北省人民医院妇科，石家庄 050051
- 折叠
摘要
Abstract
OBJECTIVE:To study the effects and mechanism of rapamycin on invasion and metastasis of cervical cancer HeLa cell. METHODS:HeLa cells were divided into control group and rapamycin low-dose,medium-dose and high-dose groups (10, 30,100 nmol/L). After treated for 48 h,cell viability was measured by MTT assay,and inhibitory rate was calculated;migration and invasion of cell was tested by Transwell assay. The expression of matrix metalloproteinase 2(MMP-2),MMP-9,Vimentin and E-cadherin,and phosphorylation of protein kinase B (Akt),mammalian target of rapamycin (mTOR) were detected by Western blot. RESULTS:Compared with control group,the inhibition rate of cell viability was increased in rapamycin groups(P<0.01);the number of invasion and metastasis cells decreased(P<0.01);the expression of MMP-2,MMP-9 and Vimentin were decreased (P<0.01 or P<0.05);the expression of E-cadherin was enhanced(P<0.01 or P<0.05);the phosphorylation of Akt and mTOR were reduced (P<0.01). CONCLUSIONS:Rapamycin could inhibit invasion and metastasis of HeLa cell via Akt/mTOR signal pathway.关键词
雷帕霉素/蛋白激酶B/雷帕霉素靶蛋白信号通路/宫颈癌HeLa细胞/侵袭/转移Key words
Rapamycin/Protein kinase B/mammalian target of rapamycin signal pathway/Cervical cancer HeLa cell/Inva-sion/Metastasis分类
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贾利刚,田菲,张媛..雷帕霉素对宫颈癌HeLa细胞侵袭转移的影响及机制研究[J].中国药房,2016,27(16):2225-2228,4.
