中国医学创新2016，Vol.13Issue(14)：1-6,6.DOI:10.3969/j.issn.1674-4985.2016.14.001
糖皮质激素在抑郁模型大鼠中对海马BDNF基因选择性剪切的影响
Effects of Glucocorticoids on the Selective Shear of BDNF Gene in the Hippocampus of Rats with Depression
摘要
Abstract
Objective:To explore the effect of glucocorticoid on the brain-derived neurotrophic factor (BDNF) gene in hippocampus of depression model rats,and to provide the theoretical basis for the etiology and treatment of depression.Method:A total of 30 male SD rats were randomly divided into the control group,high dose group and low dose group,each group had 10 rats,they were fed 5 d adaptive,the forced swimming test and open-field test were performed before administration in each group.The high and low dose group were respectively treated with oral corticosterone (CORT) 100 μg/mL and 25 μg/mL for 21 d,CORT concentration reduced to 50% of the original from 15 days,and it down to the original 25% from 18 days,the control group was treated with oral administration of 2.4% alcohol solution for 21 d. After 21 d,rats in each group were again carried on forced swimming test and exposure test and measured weight weekly,after this,for the high and low dose group, one group was selected to prepare a successful model of depression in rats and was selected as the model group. Hippocampal tissue were extracted partition,BDNF mRNA in hippocampal area were detected by using polymerase chain reaction (PCR).Result:After administration,the floating time of the high and low dose group were higher than before administration,the differences were statistically significant(P<0.05).After administration,the total movement distance,stand times,central time in high and low dose group were lower than those before administration,the differences were statistically significant(P<0.05).The body weight gain in high dose group was lower than the control group,there was no significant difference between the low dose group and the control group in the first 2 weeks,but weight gain was significantly lower than the control group at 3 week,the difference was statistically significant(P<0.05).In addition to the BDNF mRNAⅥ CA1 area and BDNF mRNAⅧ CA3 area,the transcriptional expression amount of model group in BDNF mRNAⅡa area,BDNF mRNAⅢ area, BDNF mRNAⅥ area and BDNF mRNAⅦ area were lower than those in the control group,the differences were statistically significant(P<0.05).Conclusion:Continuous oral administration of glucocorticoids can produce an ideal rat model of depression.The transcription of BDNF mRNAⅡa,BDNF mRNAⅢ,BDNF mRNAⅥ,BDNF mRNAⅦ and BDNF mRNAⅧ reduced in the decreased rats hippocampal area.关键词
抑郁症/糖皮质激素/脑源性神经营养因子/选择性剪切Key words
Depression/Glucocorticoid/Brain-derived neurotrophic factor (BDNF)/Alternative splicing引用本文复制引用
薛楠,陈建鸣,张宇,李建国,张策..糖皮质激素在抑郁模型大鼠中对海马BDNF基因选择性剪切的影响[J].中国医学创新,2016,13(14):1-6,6.基金项目
国家自然科学基金资助项目 （）
