中国医学创新2016，Vol.13Issue(14)：11-14,4.DOI:10.3969/j.issn.1674-4985.2016.14.003
齐墩果酸联合二甲亚砜对生物膜中粪肠球菌抑菌作用研究
Study of Antibacterial Effect of Oleanolic Acid Combinating with Dimethyl Sulfoxide Against Enterococcus Faecalis in Biofilm
摘要
Abstract
Objective:To evaluate the antibacterial effect of oleanolic acid(OA) and dimethyl sulfoxide (DMSO) against enterococcus faecalis (E.faecalis) in biofilm in vitro.Method:(1)The microscale checkerboard technique was applied to calculate the FICI value of OA combinating with DMSO.(2)E.faecalis biofilm models were formed after 24h. Samples were randomly divided into three groups:1 mg/mL OA+50% DMSO(group A), 2% chlorhexidine(group B), deionized water(group C).Medicating in each groups for 30 min respectively. After fluorescent staining, samples were observed by CLSM.Result:(1)The MICs of DMSO and OA against E.faecalis were 20% and 100 μg/mL.FICI=0.5125 when they were combined.(2)The biofilms of group A and B were mainly composed of dead bacteria, only scattered living bacteria could be found.The biofilm of group C was mainly composed of living bacteria,only tiny dead bacteria could be detected.Conclusion:Additive joint action was existed between OA and DMSO against E.faecalis.1 mg/mL OA combinating with 50% DMSO can effectively remove the bacteria in biofilms.关键词
齐墩果酸/二甲亚砜/粪肠球菌/激光共聚焦显微镜Key words
Oleanolic acid/Dimethyl sulfoxide/Enterococcus faecalis/Confocal laser scanning microscope引用本文复制引用
吴悠,胡焱,王瑶..齐墩果酸联合二甲亚砜对生物膜中粪肠球菌抑菌作用研究[J].中国医学创新,2016,13(14):11-14,4.基金项目
2015年四川省教育厅科研计划项目（15ZA0170）；四川省科技厅-泸州市科技局-泸州医学院联合项目 （）
