中国医学创新2016，Vol.13Issue(14)：29-32,4.DOI:10.3969/j.issn.1674-4985.2016.14.007
RGD修饰的葫芦素B纳米脂质载体对乳腺癌荷瘤裸鼠的抑瘤作用
Inhibitory Effect of RGD Modified B Nano Lipid Carrier on Breast Cancer Bearing Nude Mice
摘要
Abstract
Objective:To prepare RGD conjugated cucurbitacin B loaded liposome(RGD-CuB-NLC) and observe RGD-CuB-NLC’s anti-cancer effect on the treatment with the cells of breast cancer in vitro and RGD-CuB-NLC’s anti-cancer effect on nude mice of human breast cancer.Method:The liposomes were prepared by thin film hydration method,a single cell suspension liquid of the MCF-7 cells of breast cancer was made according to the cultivation of the adherent cell method,then it was inoculated into 96 hole cell culture plate,the group of RGD-CuB-NLC was designed,the control groups were CuB-NLC,RGD-NLC and the blank group of NLC were designed,the rate of suppression in different condition were calculated.Nude mouse transplantation tumor were established,30 qualified nude mice models of human breast cancer xenograft were randomly divided into 5 groups, each group was 6,the mice of 5 groups were respectively treated by CuB,CuB-NLC,RGD-NLC,RGD-CuB-NLC and 0.9% sodium chloride solution every 24 hours per time,7 times in total.The body weight of nude mice and the weight of the transplanted tumor growth were recorded,and the inhibition rate of tumor growth were calculated. Result:For RGD-CuB-NLC group,the form was the circular,the encapsulation rate>80%,partical size was less than 150 nm,the average particle size was (115.6±2.1)nm,polydispersity index was (0.217±0.033),the electric potential was(-12.31±0.76)mV.The inhibition ratio of different liposome on breast cancer cells in the group of NLC was 0,the group of CuB-NLC was (46.3±2.21)%,the group of RGD-NLC was (55.7±3.22)%, the group of RGD-CuB-NLC was (75.6±5.67)%,the differences were statistically significant(P<0.05). In the inhibition rate of tumor growth,there were statistically significant in 0.9% sodium chloride solution group, CuB group,CuB-NLC group and RGD-NLC group compared with RGD-CuB-NLC group(P<0.05).There were statistically significant in CuB group,CuB-NLC group,RGD-NLC group and RGD-CuB-NLC group compared with 0.9% sodium chloride solution group in the inhibition rate of tumor growth(P<0.05).Conclusion:RGD conjugated cucurbitacin B loaded liposome can restrain the proliferation of breast cancer cells and the growth of tumor.关键词
RGD肽/葫芦素B/纳米脂质载体/乳腺癌/抑瘤Key words
RGD peptide/Cucurbitacin B/Nanostructured lipid carriers/Cancer of breast/Antitumor引用本文复制引用
唐淑洁,赵旭伟,张佳琦,刘洋,王梓丞..RGD修饰的葫芦素B纳米脂质载体对乳腺癌荷瘤裸鼠的抑瘤作用[J].中国医学创新,2016,13(14):29-32,4.基金项目
齐齐哈尔市科技局科学技术计划项目 （）
