中国药业2016，Vol.25Issue(7)：30-32,3.
高效液相色谱法测定人血清中茶碱浓度
Determination of Serum Theophylline Concentration by HPLC
孔幸珊 1曾彩芳 1杨佳彬1
作者信息
- 1. 广州医科大学附属第四医院，广东 广州 511447
- 折叠
摘要
Abstract
Objective To establish an HPLC method to determine the concentration of theophylline in human plasma. Methods The serum sample was performed the precipitation treatment by 10% trifluoroacetic acid , the chromatographic column was the Inertsil ODS-SP column ( 250 mm × 4. 6 mm, 5 μm ) , the mobile phase was consisted of methanol-water ( 25 :75 ) . The UV detection wavelength was at 274 nm with a flow rate of 1. 0 mL/min and the column temperature of 25 ℃. Results The concentration of theophylline in the range of 2. 55-40. 72 μg/mL showed good linearity. The regression equation of plasma theophylline concentration and peak area was Y=( 45 417. 2 ) X-4 580. 0, r=0. 999 8 ( n=6 ) . The intra-day and inter-day RSD were less than 4. 88% and 6. 06%, and the limit quantitation was 0. 2 μg/mL. Conclusion The established method is fast, accurate and suitable for therapeutic dug monitoring ( TDM ) of theophylline in clinical.关键词
茶碱/高效液相色谱法/血药浓度监测Key words
theophylline/HPLC/TDM分类
医药卫生引用本文复制引用
孔幸珊,曾彩芳,杨佳彬..高效液相色谱法测定人血清中茶碱浓度[J].中国药业,2016,25(7):30-32,3.
